https://eln.excellgen.com/index.php?action=history&feed=atom&title=3237_pCMV5-%28Tev%29-Avi-6H-BirA 3237 pCMV5-(Tev)-Avi-6H-BirA - Revision history 2026-10-19T04:33:01Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=3237_pCMV5-(Tev)-Avi-6H-BirA&diff=18379&oldid=prev Paul.xu at 16:09, 23 June 2018 2018-06-23T16:09:44Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039;&amp;nbsp;&amp;nbsp;<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2018-6-22<br /> <br /> &#039;&#039;&#039;Notebook:&amp;nbsp;&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Clone ID:3237&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp;Paul&amp;nbsp;<br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === &#039;&#039;&#039;PCR&#039;&#039;&#039; ===<br /> <br /> Date:&amp;nbsp;Date: 6/21/2018<br /> <br /> By: Paul<br /> <br /> template:<br /> <br /> primers:<br /> <br /> Gel:<br /> <br /> http://www.excellgen.net/eln/images/5/52/3237_3232_3234_PCR.JPG<br /> <br /> <br /> <br /> &#039;&#039;&#039;Cloning&#039;&#039;&#039;<br /> <br /> By: Paul<br /> <br /> Vector:&amp;nbsp;&amp;nbsp;<br /> <br /> Ligation: 30 min with T4 DNA ligase<br /> <br /> Competent cells: NEBTurbo<br /> <br /> <br /> <br /> &#039;&#039;&#039;Colony PCR Screening&amp;nbsp;&#039;&#039;&#039;<br /> <br /> 6/23/18<br /> <br /> <br /> <br /> [[File:3237 3232 3234 minipre digestion.JPG]]<br /> <br /> <br /> <br /> <br /> <br /> &#039;&#039;&#039;Restriction Digestion&#039;&#039;&#039;<br /> <br /> Date: 6/~/2018<br /> <br /> By: Paul<br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> &#039;&#039;&#039;Sequencing&#039;&#039;&#039;<br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> [[Category:Cloning]]</div> Paul.xu