https://eln.excellgen.com/index.php?action=history&feed=atom&title=3248_pQE-80L-T7-RNAP-P266L 2026-10-18T10:42:25Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=3248_pQE-80L-T7-RNAP-P266L&diff=19114&oldid=prev 2018-08-23T17:47:11Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 3248 pQE-80L-T7-RNAP-P266L&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 3248 pQE-80L-T7-RNAP-P266L<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;07, 30, 2018<br /> <br /> &#039;&#039;&#039;Notebook:&amp;nbsp;&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Clone ID: 3248&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Experimenters: &#039;&#039;&#039;paul<br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Cloning ===<br /> <br /> === &amp;nbsp;Colony screen ===<br /> <br /> &#039;&#039;&#039;3248 miniprep digestion 1&#039;&#039;&#039;<br /> <br /> [[File:IMG 1240.JPG|RTENOTITLE]]<br /> <br /> &#039;&#039;&#039;3248 miniprep digestion 2&#039;&#039;&#039;<br /> <br /> [[File:IMG 1243.JPG|RTENOTITLE]]<br /> <br /> 8/9/2018<br /> <br /> [[File:3248 fix.JPG|RTENOTITLE]]<br /> <br /> 8/21/2018<br /> <br /> &#039;&#039;&#039;1st PCR fix of 3248&#039;&#039;&#039;<br /> <br /> [[File:IMG 1320.JPG|RTENOTITLE]]<br /> <br /> &#039;&#039;&#039;2nd PCR fix of 3248&#039;&#039;&#039;<br /> <br /> [[File:IMG 1321.JPG|RTENOTITLE]]<br /> <br /> Vector has already been prepared by Paul<br /> <br /> Enzymes sites:Kpn1+HindIII<br /> <br /> &#039;&#039;&#039;8/23/2018 miniprep&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Kpn1+HindIII&#039;&#039;&#039;<br /> <br /> [[File:IMG 1326.JPG|RTENOTITLE]]<br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> [[Category:Cloning]]</div>

Crm