https://eln.excellgen.com/index.php?action=history&feed=atom&title=3255_pCMV5-myc-rk 3255 pCMV5-myc-rk - Revision history 2026-10-18T11:23:04Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=3255_pCMV5-myc-rk&diff=19069&oldid=prev Crm at 18:12, 20 August 2018 2018-08-20T18:12:09Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 3255 pCMV5-myc-rk&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 3255 pCMV5-myc-rk<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;08, 15, 2018<br /> <br /> &#039;&#039;&#039;Notebook:&amp;nbsp;&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Clone ID: 3255&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Experimenters: &#039;&#039;&#039;yijia<br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Cloning ===<br /> <br /> PCR from 3213 pANT7_cGST-MICA<br /> <br /> &#039;&#039;&#039;1st PCR of 3255&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;template:&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;F1:3075&#039;&#039;&#039; PCR from 3075 pCMV5-Myc-L-kappa&lt;br/&gt;3255-EcoR1-F1 TGTCACGAATTCAgacatcgtgctgacacagag&amp;nbsp;<br /> <br /> &#039;&#039;&#039;F2:1414&#039;&#039;&#039; PCR&amp;nbsp; from 1414 pCDNA3.4-Rabbit-IgG Light Chain&lt;br/&gt;3255-F2&amp;nbsp; gccggaacaaagctggaaatcaagGCTAGCGGTACCGATCCAGTTGCACCTACTG<br /> <br /> &#039;&#039;&#039;F3:1414&#039;&#039;&#039; PCR from 1414 pCDNA3.4-Rabbit-IgG Light Chain&lt;br/&gt;3255-F3 CACCGCAGAACTCTGCAGATTGTAC<br /> <br /> [[File:IMG 1303.JPG|RTENOTITLE]]<br /> <br /> &#039;&#039;&#039;2nd PCR of 3255&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Template:combine F1+F2+F3&#039;&#039;&#039; 3255-EcoR1-F1 TGTCACGAATTCAgacatcgtgctgacacagag&amp;nbsp;<br /> <br /> [[File:IMG 1307.JPG|RTENOTITLE]]<br /> <br /> &#039;&#039;&#039;Vector digestion&#039;&#039;&#039;<br /> <br /> Vector is 3075<br /> <br /> [[File:IMG 1304.JPG|RTENOTITLE]]<br /> <br /> === &amp;nbsp;Colony screen ===<br /> <br /> [[File:IMG 1313.JPG|RTENOTITLE]]<br /> <br /> &#039;&#039;&#039;Miniprep of 3255&#039;&#039;&#039;<br /> <br /> {| border=&quot;1&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; style=&quot;width:500px;&quot;<br /> |-<br /> | #<br /> | Lane<br /> | Enzymes<br /> | Length<br /> | Buffer<br /> | Note<br /> |-<br /> | 3255<br /> | 5-8(#1-4)<br /> | EcoR1+Not1<br /> | 671bp<br /> | Fastdigest 30min<br /> | #4 to do sequence<br /> |-<br /> | vector control<br /> | 3075<br /> | EcoR1+Not1<br /> | 685bp<br /> | Fastdigest 30min<br /> | &lt;br/&gt;<br /> |}<br /> <br /> [[File:IMG 1316.JPG|RTENOTITLE]]<br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> [[Category:Cloning]]</div> Crm