https://eln.excellgen.com/index.php?action=history&feed=atom&title=3256_pCMV5-myc-rIgG 3256 pCMV5-myc-rIgG - Revision history 2026-10-18T07:30:05Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=3256_pCMV5-myc-rIgG&diff=19072&oldid=prev Crm at 18:15, 20 August 2018 2018-08-20T18:15:38Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 3256 pCMV5-myc-rIgG&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 3256 pCMV5-myc-rIgG<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;08, 15, 2018<br /> <br /> &#039;&#039;&#039;Notebook:&amp;nbsp;&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Clone ID: 3256&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Experimenters: &#039;&#039;&#039;yijia<br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Cloning ===<br /> <br /> &#039;&#039;&#039;1st PCR of 3256&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;F1:&#039;&#039;&#039; PCR from 3167 pCEH0-Myc-VH-mIgG2a&lt;br/&gt;3256-EcoR1-F1 TGTCACGAATTCAGAGGTGGACCTGGTTGAGTCTG<br /> <br /> &#039;&#039;&#039;F2:&#039;&#039;&#039; PCR&amp;nbsp; from 1413 pCDNA3.4-Rabbit-IgG Heavy Chain&lt;br/&gt;3256-F2&amp;nbsp; CACCACAGTGACCGTTTCTTCTGCTAGCGGTACCGGGCAACCTAAGGCTCCATC<br /> <br /> [[File:IMG 1303.JPG|RTENOTITLE]]<br /> <br /> &#039;&#039;&#039;2nd PCR of 3256&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Template:combine F1+F2&#039;&#039;&#039; 3256-EcoR1-F1 TGTCACGAATTCAGAGGTGGACCTGGTTGAGTCTG<br /> <br /> [[File:IMG 13082.JPG|RTENOTITLE]]<br /> <br /> &#039;&#039;&#039;Vector digestion&#039;&#039;&#039;<br /> <br /> Vector is 3075<br /> <br /> [[File:IMG 1304.JPG|RTENOTITLE]]<br /> <br /> === &amp;nbsp;Colony screen ===<br /> <br /> [[File:IMG 1314.JPG|RTENOTITLE]]<br /> <br /> &#039;&#039;&#039;Miniprep of 3256&#039;&#039;&#039;<br /> <br /> {| border=&quot;1&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; style=&quot;width:500px;&quot;<br /> |-<br /> | #<br /> | Lane<br /> | Enzymes<br /> | Length<br /> | Note<br /> |-<br /> | 3256<br /> | 9-12(#1-4)<br /> | EcoR1+Not1<br /> | 1380bp<br /> | #2 to do sequence<br /> |-<br /> | vector control<br /> | 3075<br /> | EcoR1+Not1<br /> | 685bp<br /> | &lt;br/&gt;<br /> |}<br /> <br /> <br /> <br /> [[File:IMG 1316.JPG|RTENOTITLE]]<br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> [[Category:Cloning]]</div> Crm