https://eln.excellgen.com/index.php?action=history&feed=atom&title=3258_pCMV5-anti-mIgGFc-rFc-His 3258 pCMV5-anti-mIgGFc-rFc-His - Revision history 2026-10-18T08:54:37Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=3258_pCMV5-anti-mIgGFc-rFc-His&diff=19450&oldid=prev Paul.xu at 18:47, 15 October 2018 2018-10-15T18:47:00Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 3258 pCMV5-anti-mIgGFc-rFc-His&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 3258 pCMV5-anti-mIgGFc-rFc-His<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;08, 15, 2018<br /> <br /> &#039;&#039;&#039;Notebook:&amp;nbsp;&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Clone ID: 3258&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Experimenters: &#039;&#039;&#039;yijia<br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Cloning ===<br /> <br /> &#039;&#039;&#039;gene synthesis of 3258&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;template:&#039;&#039;&#039;<br /> <br /> gene&amp;nbsp; synthesis<br /> &lt;div&gt;3258-1TGTCACGAATTCACAGGTGCAGCTGGTTGAATCTGGCGGAG&lt;/div&gt;&lt;div&gt;3258-2CGGCACAATACAGTCTCAGGCTTCCGCCAGGCTGAACCAGTCCTCCGCCAGATTCAACCA&lt;/div&gt;&lt;div&gt;3258-3CCTGAGACTGTATTGTGCCGCCAGCGGCAGAACCGACACAACATATGCCCTCGGCTGGTT&lt;/div&gt;&lt;div&gt;3258-4GATGCTGGCCACGAACTGTCTTTCTTTGCCAGGAGGCTGTCTGAACCAGCCGAGGGCATA&lt;/div&gt;&lt;div&gt;3258-5CAGTTCGTGGCCAGCATCACCTGGATCGGCGGAGCCACAAATTACGCCGCCTCTGTGAAG&lt;/div&gt;&lt;div&gt;3258-6TGAATGTGTTGGTGCCCAGGTCCTTGCTGATGGTGAATCTGCCCTTCACAGAGGCGGCGT&lt;/div&gt;&lt;div&gt;3258-7CTGGGCACCAACACATTCAACCTGCAGATGAACAGCCTGACACCTGACGACACCGCCGTG&lt;/div&gt;&lt;div&gt;3258-8TGAGCATTGGGCCACTGTCTAACGACGGCGGCAGCACAATAGTACACGGCGGTGTCGTCA&lt;/div&gt;&lt;div&gt;3258-9ACAGTGGCCCAATGCTCATCAGGGCGCCTACGATTATTGGGGCCAGGGCACACAAGTGAC&lt;/div&gt;&lt;div&gt;3258-10CTTGCTGGTACCGCTAGCACTAGACACGGTCACTTGTGTGCCCTGG&lt;/div&gt;&lt;div&gt;digest with EcoR1 + Nhe1 or EcoR1+ Kpn1<br /> [[File:IMG 1302.JPG|RTENOTITLE]]<br /> <br /> <br /> <br /> &#039;&#039;&#039;Vector digestion&#039;&#039;&#039;<br /> <br /> Fastdigest&amp;nbsp; 1 hour EcoR1+Kpn1<br /> <br /> Vector is 3257 [[File:IMG 1333.JPG|RTENOTITLE]]<br /> <br /> &#039;&#039;&#039;minprep of 3258&#039;&#039;&#039;<br /> <br /> [[File:IMG 2049.JPG|RTENOTITLE]]<br /> <br /> === &amp;nbsp;Colony screen ===<br /> <br /> &#039;&#039;&#039;3258 PCR Screening&#039;&#039;&#039;<br /> <br /> [[File:IMG 1336.JPG|RTENOTITLE]]<br /> <br /> &lt;span style=&quot;font-size:larger;&quot;&gt;&#039;&#039;&#039;Pick up#1,#3,#4,#6 to grow up o/n to do miniprep digestion&#039;&#039;&#039;&lt;/span&gt;<br /> <br /> &#039;&#039;&#039;3258 Miniprep digestion&#039;&#039;&#039;<br /> <br /> [[File:IMG 1339.JPG|RTENOTITLE]]<br /> <br /> &lt;span style=&quot;font-size:large;&quot;&gt;Failed, might be empty vector&lt;/span&gt;<br /> <br /> 9/3/2018, digest miniprep DNA with EcoR1-Kpn1<br /> <br /> &lt;span style=&quot;font-size:large;&quot;&gt;[[File:3258 EcoR1 Kpn1.JPG|RTENOTITLE]]&lt;/span&gt;<br /> <br /> &#039;&#039;&#039;3258 PCR SCreening&#039;&#039;&#039;<br /> <br /> [[File:IMG 1392.JPG|RTENOTITLE]]<br /> <br /> &#039;&#039;&#039;3258 Miniprep digestion&#039;&#039;&#039;<br /> <br /> EcoR1+Kpn1<br /> <br /> [[File:IMG 1395.JPG|RTENOTITLE]]<br /> <br /> [[File:3261 3258 3260 3262 3259.JPG]]<br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> &lt;/div&gt;[[Category:Cloning]]</div> Paul.xu