https://eln.excellgen.com/index.php?action=history&feed=atom&title=3272_pCMV-IRES-SVLT 3272 pCMV-IRES-SVLT - Revision history 2026-10-18T17:19:14Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=3272_pCMV-IRES-SVLT&diff=19308&oldid=prev Crm at 16:41, 24 September 2018 2018-09-24T16:41:14Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 3272 pCMV-IRES-SVLT&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose: Cloning of 3272 pCMV-IRES-SVLT&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;08, 29, 2018<br /> <br /> &#039;&#039;&#039;Notebook: 008840&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Clone ID: 3272&#039;&#039;&#039; &#039;&#039;&#039;Experimenters: &#039;&#039;&#039;yijia<br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Cloning ===<br /> <br /> &#039;&#039;&#039;subclone digestion&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;vector is 3273;Not11+Pme1&#039;&#039;&#039;<br /> <br /> [[File:IMG 1338.JPG|RTENOTITLE]]<br /> <br /> === &amp;nbsp;Colony screen ===<br /> <br /> &#039;&#039;&#039;Miniprep of 3272&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Enzymes:Xba1+Kpn1&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Size:2644bp ,438bp&#039;&#039;&#039;<br /> <br /> [[File:IMG 1344.JPG|RTENOTITLE]]<br /> <br /> #send #1 to do sequence<br /> <br /> &#039;&#039;&#039;Miniprep digestion 3272&#039;&#039;&#039;<br /> <br /> [[File:IMG 1373.JPG|RTENOTITLE]]<br /> <br /> &#039;&#039;&#039;PCR SCreening&#039;&#039;&#039;<br /> <br /> [[File:IMG 1375.JPG|RTENOTITLE]]<br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> [[Category:Cloning]]</div> Crm