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344-DsbA-PET30b - Revision history
2026-10-18T08:04:17Z
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Luyingying at 17:09, 7 October 2011
2011-10-07T17:09:11Z
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<p><b>New page</b></p><div>Cloning of DsbA by Gene Synthesis <br />
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Purpose: to clone DsbA-PET30b<br> <br />
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Date: 2011-9-27 <br />
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Notebook: luyingying <br />
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Page: <br />
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Clone ID:344 <br />
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Experimenters: lu yingying <br />
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Result <br />
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[[Image:344DsbA-PCR product-9-27-2011.JPG|331x163px|344DsbA-PCR product-9-27-2011.JPG]]<br> <br />
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template:gene synthesis DsbA1 to DsbA18<br> <br />
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PrimerF:DsbA1,PrimerR:DsbA18<br> <br />
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[[Image:344-382-9-27-2011.JPG|382x202px|344-382-9-27-2011.JPG]]<br> <br />
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digest result:Nde1+Kpn1<br> <br />
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Band 1,2 show DsbA digest with 10*fast digetion buffer,Band 3 shows PET30b digest with 10*fast digetion buffer<br> <br />
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Band 4 show DsbA digest with 10*NEB(1) buffer,band 5 shows PET30b digest with 10*NEB(1) buffer<br> <br />
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[[Image:344-345-382-digest detecting result9-29-2011.JPG|509x149px|344-345-382-digest detecting result9-29-2011.JPG]] <br />
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no result <br />
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<br>Gene Synthesis <br />
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Colony Screening <br> <br />
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Restart:<br> <br />
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Date: 2011-10-4 <br />
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Notebook: luyingying <br />
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Page: <br />
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Clone ID:344 <br />
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Experimenters: lu yingying <br />
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Result <br />
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[[Image:344-DsbA-PCR product10-4-2011.JPG]] <br />
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All band show DsbA PCR product<br> <br />
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template:gene synthesis DsbA1 to DsbA18 <br />
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PrimerF:DsbA1,PrimerR:DsbA18<br> <br />
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[[Image:344DsbA+PET30b digest 10-5-2011.JPG]]<br> <br />
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digest result:Nde1+Kpn1 <br />
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Band 1 shows DsbA digest with 10*fast digetion buffer,Band 2 shows PET30b digest with 10*fast digetion buffer<br> <br />
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[[Image:344,359,391+393digest detect10-7-2011.JPG|539x250px|344,359,391+393digest detect10-7-2011.JPG]] <br />
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344,1-6 bands show digest result: no insert <br />
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[[Category:Cloning]]</div>
Luyingying