https://eln.excellgen.com/index.php?action=history&feed=atom&title=372-pAd5-CMV-PKM2 372-pAd5-CMV-PKM2 - Revision history 2026-10-18T11:51:50Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=372-pAd5-CMV-PKM2&diff=1580&oldid=prev Paul.xu at 22:34, 31 August 2011 2011-08-31T22:34:30Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Construction of pAd5-CMV-PKM2 372&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; construction of pAd5-CMV-PKM2 (372). <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2011-8-31 to 2011-9- <br /> <br /> &#039;&#039;&#039;Notebook:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; Paul&lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Digestion&lt;br&gt; ===<br /> <br /> Date: 2011-8-31 <br /> <br /> Digest &amp;nbsp;pET3a-Muts (clone #352) with Nde1+ Sph1&lt;br&gt; <br /> <br /> Digest&amp;nbsp; pDB.His.MBP (clone # 360 ) with Nde1+ Sph1&lt;br&gt; <br /> <br /> cut out insert MBP and vector pET3a-Muts , ligate and transform Top10&lt;br&gt; <br /> <br /> [[Image:PKM2 Muts 8 31 2011.JPG]] <br /> <br /> --[[User:Paul.xu|Paul.xu]] 13:02, 31 August 2011 (EST) <br /> <br /> [[Image:PKM2 PCR digestion.jpg]]&lt;br&gt; <br /> <br /> digest PCR product and vector in one tube with BamH1 + Xho1, run gel and purify, ligate, transform stbl3. <br /> --[[User:Paul.xu|Paul.xu]] 17:34, 31 August 2011 (EST)<br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2011-9-1&lt;br&gt; &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> Restriction digestion &lt;br&gt; <br /> <br /> Shows&amp;nbsp; &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div> Paul.xu