https://eln.excellgen.com/index.php?action=history&feed=atom&title=374-pAd5.CMV-Dest-His
374-pAd5.CMV-Dest-His - Revision history
2026-10-18T11:08:19Z
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https://eln.excellgen.com/index.php?title=374-pAd5.CMV-Dest-His&diff=2557&oldid=prev
Luyingying at 21:36, 23 November 2011
2011-11-23T21:36:57Z
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<p><b>New page</b></p><div>Cloning of 374-pAd5.CMV-Dest-His <br />
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Purpose: to clone pAd5.CMV-Dest-His by gene synthesis<br> <br />
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Date: 2011-11-9---- <br />
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Notebook:Lu yingying <br />
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Page: <br />
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Clone ID:374 <br />
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Experimenters: lu yingying<br>Results<br> <br />
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[[Image:374 PCR 11-9-2011.JPG]]<br> <br />
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PCR gateway cassette using 446-pVP16 vector as template<br> <br />
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ad-GW-F,ad-GW-His-R <br />
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[[Image:374 clone 11-9-2011.JPG]]<br>cut with Kpn1 + Xho1<br>ligate to Kpn1 +Xho1 digested vector280<br> <br />
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Date: 2011-11-15---- <br />
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Notebook:Lu yingying <br />
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Page: <br />
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Clone ID:374 <br />
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Experimenters: lu yingying<br>Results<br> <br />
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[[Image:374 PCR 11-15-2011.JPG]]<br> <br />
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PCR gateway cassette using 446-pVP16 vector as template <br />
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ad-GW-F,ad-GW-His-R <br> <br />
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[[Image:374 digest11-15-2011.JPG]]<br> <br />
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cut with Kpn1 + Xho1<br>ligate to Kpn1 +Xho1 digested vector280<br> <br />
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[[Image:374 digest detect11-17-2011.JPG]]<br> <br />
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5 different clones digest detect:Kpn1 + Xho1<br> <br />
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[[Image:393+374 test11-21-2011.JPG]] <br />
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digest&nbsp;:BamH1 <br />
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24 hours after transfection<br />
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[[Image:237 374 s 11.23.2011.jpg]]<br> <br />
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[[Image:237 374 s 11.23.2011 blue.JPG]]<br> <br />
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[[Category:Cloning]]</div>
Luyingying