https://eln.excellgen.com/index.php?action=history&feed=atom&title=380-pCMVK-IL2-Fc 380-pCMVK-IL2-Fc - Revision history 2026-10-18T08:48:03Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=380-pCMVK-IL2-Fc&diff=1939&oldid=prev Luyingying at 19:17, 5 October 2011 2011-10-05T19:17:36Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;SubCloning of CFP from pet28b-cfp to the pAAV-MCS (254)&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; SubCloning of CFP from pet28b-cfp to the pAAV-MCS (254). <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2011-8-3 to 2011-8-10 <br /> <br /> &#039;&#039;&#039;Notebook:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; Robert, Mindy, Yolanda&lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Subcloning ===<br /> <br /> Date: 2011-8-4 <br /> <br /> Digest&amp;nbsp; (clone #) with &lt;br&gt; <br /> <br /> Digest&amp;nbsp; (clone #) with &lt;br&gt; <br /> <br /> cut out insert and vector, ligate and transform &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2011-8-10&lt;br&gt; <br /> <br /> Make miniprep and digest with BamH1 + Xho1&lt;br&gt; <br /> <br /> [[Image:PAAV-mkate2 2011 8 10.jpg]]&lt;br&gt; <br /> <br /> &amp;nbsp;Lane 1: 1 kb ladder&lt;br&gt; <br /> <br /> &amp;nbsp;Lanes 2-6: pAAV-mkate2 <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *pAAV-mkate2 clones &lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> Restriction digestion &lt;br&gt; <br /> <br /> Shows&amp;nbsp; &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Cloning of&amp;nbsp; pCMVK-IL2-Fc&#039;&#039;&#039;&lt;br&gt; ==<br /> <br /> Purpose: Cloning of intfc&amp;nbsp; to the pCMVK-IRES-TagGFP(382)&lt;br&gt; <br /> <br /> Date: 2011-10-03&lt;br&gt; <br /> <br /> Notebook: <br /> <br /> Page: <br /> <br /> Clone ID: <br /> <br /> Experimenters: lu yingying, wangxiaojing&lt;br&gt; <br /> <br /> Result:&lt;br&gt; <br /> <br /> [[Image:380intfc+PCMVK-TagGFP-digest result10-3-2011.JPG]]&lt;br&gt; <br /> <br /> enzyme digest result:Sal1+ HindIII&lt;br&gt; <br /> <br /> band 1 shows pCMVK-TagGFP vector&lt;br&gt; <br /> <br /> band2,3 show intFc <br /> <br /> [[Image:380_digest_detect10-5-2011.JPG]]&lt;br&gt;<br /> <br /> digest detect:Sal1+HindIII&lt;br&gt;<br /> <br /> 6 different clones<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div> Luyingying