https://eln.excellgen.com/index.php?action=history&feed=atom&title=414-pAd5-CMV-Cre 414-pAd5-CMV-Cre - Revision history 2026-10-18T08:46:36Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=414-pAd5-CMV-Cre&diff=2890&oldid=prev Yingying.lu at 19:56, 29 December 2011 2011-12-29T19:56:23Z <p></p> <p><b>New page</b></p><div>Cloning of pAd5-CMV-Cre&lt;br&gt; <br /> <br /> Purpose: to clone pAd5-CMV-Cre by subclone from241 pLentiGP-Cre to 280 pAd5-CMV-MCS&lt;br&gt; <br /> <br /> Date: 2011-9-29 <br /> <br /> Notebook:Lu yingying <br /> <br /> Page: <br /> <br /> Clone ID:414 <br /> <br /> Experimenters: lu yingying&lt;br&gt;Results&lt;br&gt; <br /> <br /> [[Image:256,414 subclone9-29-2011.JPG]] <br /> <br /> bands 3 show cre insert ,bands 4 show pAd5 vector <br /> <br /> Ligation at RT for 2h <br /> <br /> [[Image:256,414,417digest detect10-3-2011.JPG|457x225px|256,414,417digest detect10-3-2011.JPG]]&lt;br&gt; <br /> <br /> digest detect:EcoR1+Xho1&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> Date: 2011-10-25 <br /> <br /> Notebook:Lu yingying <br /> <br /> Page: <br /> <br /> Clone ID:414 <br /> <br /> Experimenters: lu yingying&lt;br&gt;Results&lt;br&gt; <br /> <br /> [[Image:414+375 subclone digest10-25-2011.JPG]]&lt;br&gt; <br /> <br /> band 1,2 show subclone digest result:EcoR1+Xho1&lt;br&gt; <br /> <br /> 241 pLentiGP-Cre to 280 pAd5-CMV-MCS, band 1 show 241 digest result,band 2 show 280 digest result&lt;br&gt; <br /> <br /> [[Image:414+470+375+359 digest detect10-27-2011.JPG|494x270px|414+470+375+359 digest detect10-27-2011.JPG]]&lt;br&gt; <br /> <br /> band 414,1-6 show digest detect result:EcoR1+Xho1&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Cloning of pAd5-CMV-Cre&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of pAd5-CMV-Cre&amp;nbsp; from241 pLentiGP-Cre to 280 pAd5-CMV-MCS <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2011-12-14--- <br /> <br /> &#039;&#039;&#039;Notebook:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID: 414&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Subcloning ===<br /> <br /> Date: 2011-12-14--- <br /> <br /> [[Image:414,454 Clone 12-14-2011.JPG]]&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; style=&quot;width: 301px; height: 80px;&quot;<br /> |-<br /> | 414&lt;br&gt; <br /> | band1&lt;br&gt; <br /> | band2&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | 241 pLentiGP-Cre&lt;br&gt; <br /> | 280 pAd5-CMV-MCS&lt;br&gt;<br /> |-<br /> | digest&lt;br&gt; <br /> | EcoR1+Xho1&lt;br&gt; <br /> | EcoR1+Xho1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2011-12-16&lt;br&gt; <br /> <br /> Make miniprep and digest with EcoR1+Xho1&lt;br&gt; <br /> <br /> &amp;nbsp;[[Image:414+454 digest detect 12-16-2011.JPG]]&lt;br&gt; <br /> <br /> lanes 1,2,3,5,6,7 show right, need to sequence&lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *Sequencing Result:414-6 right&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> 414-6 right<br /> &lt;br&gt; &lt;br&gt; ==<br /> <br /> === ===<br /> <br /> &lt;br&gt; --[[User:Yingying.lu|Yingying.lu]] 08:47, 19 December 2011 (EST) <br /> <br /> [[Category:Cloning]]</div> Yingying.lu