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417-pAAV-iRFP - Revision history
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Luyingying at 20:02, 3 October 2011
2011-10-03T20:02:43Z
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<p><b>New page</b></p><div>Cloning of pAAV-iRFP<br> <br />
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Purpose: to clone&nbsp; pAAV-iRFP by subclone from 338 pET28b-iRFP to 254 pAAV-MCS<br> <br />
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Date: 2011-9-30 <br />
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Notebook:Lu yingying <br />
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Page: <br />
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Clone ID:417 <br />
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Experimenters: lu yingying<br>Results<br> <br />
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[[Image:338,254,280,152digest result9-29-2011.JPG|486x263px|338,254,280,152digest result9-29-2011.JPG]]<br> <br />
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digest result: BamH1+Xho1<br> <br />
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bands 1,3,5,7,9,11 show IRFP&nbsp; insert <br> <br />
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band 2 shows PAAV vector<br> <br />
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Ligation at RT for 2h <br />
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[[Image:256,414,417digest_detect10-3-2011.JPG|563x277px]]<br><br />
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digest detect:BamH1 +Xho1<br> <br />
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[[Category:Cloning]]</div>
Luyingying