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418-pAd.CMV-iRFP - Revision history
2026-10-18T16:37:46Z
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Luyingying at 19:25, 15 November 2011
2011-11-15T19:25:43Z
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<p><b>New page</b></p><div>Cloning of pAd5-CMV-iRFP <br />
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Purpose: to clone pAd5-CMV-iRFP by subclone from 338&nbsp;pET28b-iRFP to&nbsp; 280 pAd5-CMV-MCS <br />
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Date: 2011-11-7----2011-11-10 <br />
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Notebook:Lu yingying <br />
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Page:003968-----003976 <br />
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Clone ID:418 <br />
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Experimenters: lu yingying<br>Results<br> <br />
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[[Image:418+419 subclone 11-8-2011.JPG]]<br> <br />
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digest:BamH1+Xho1<br> <br />
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band 1 show insert ,band 2 show vector<br> <br />
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[[Image:418+419+456 digest detect11-10-2011.JPG]]<br> <br />
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5 clonies digest detect:BamH1+Xho1,band 3 ,5 may be right, need&nbsp; to sequence<br> <br />
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[[Category:Cloning]]</div>
Luyingying