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2026-10-18T14:12:57Z
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Yingying.lu at 18:00, 9 December 2011
2011-12-09T18:00:08Z
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<p><b>New page</b></p><div>Cloning of pET30b-sso7d-DeepVent <br />
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Purpose: to clonepET30b-sso7d-DeepVent by PCR <br />
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Date: 2011-12-8 <br />
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Notebook:Lu yingying <br />
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Page: <br />
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Clone ID:422 <br />
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Experimenters: lu yingying<br>Results <br />
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[[Image:422+423_PCR_12-9-2011.JPG]]<br><br />
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PCR using NEB720 Deep Vent (385) as template<br>to clone into pET30b-sso7d-NK 369 <br>DVent-Nco1-F<br>atcgaa CC ATGGtacttgacgctgactac<br />
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DVent-Sal1-R <br />
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(ggcttaacatcaagaagaag GTCGAC atcaga)<br>tctgatGTCGACcttcttcttgatgttaagcc<br><br />
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--[[User:Yingying.lu|Yingying.lu]] 13:00, 9 December 2011 (EST)<br />
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[[Category:Cloning]]</div>
Yingying.lu