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457-PET28b-CymR - Revision history
2026-10-18T16:04:41Z
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Yingying.lu at 21:11, 8 December 2011
2011-12-08T21:11:20Z
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<p><b>New page</b></p><div>Cloning of 457-PET28b-CymR <br />
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Purpose:&nbsp; gene synthesis&nbsp; to cloning of 457-PET28b-CymR<br> <br />
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Date: 2011-11-28---- <br />
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Notebook:Lu yingying <br />
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Page: <br />
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Clone ID:457 <br />
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Experimenters: lu yingying<br>Results<br> <br />
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[[Image:457 PCR 11-29-2011.JPG]]<br> <br />
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Gene synthesis<br> <br />
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template: CymR1 to CymR18 mixture <br />
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&nbsp;CymR 1 and CymR18 as primers <br />
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[[Image:457+461 clone 11-29-2011.JPG]]<br> <br />
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digest:BamH1+ Xho1<br> <br />
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band 1 show insert ,band 2 show PET28b vector <br />
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Date: 2011-12-6---- <br />
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Notebook:Lu yingying <br />
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Page: <br />
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Clone ID:457 <br />
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Experimenters: lu yingying<br>Results<br> <br />
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[[Image:457+471+477+500 PCR 12-5-2011.JPG|406x168px|457+471+477+500 PCR 12-5-2011.JPG]]<br> <br />
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Gene synthesis <br />
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template: CymR1 to CymR18 mixture <br />
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CymR 1 and CymR18 as primers <br> <br />
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[[Image:457+471 clone 12-6-2011.JPG]]<br> <br />
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digest:BamH1+ Xho1 <br />
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band 1 show insert ,band 2 show PET28b vector <br> <br />
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[[Image:457+491_digest_detect_12-8-2011.JPG]]<br><br />
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digest detect: BamH1+ Xho1 <br> <br />
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[[Category:Cloning]]</div>
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