https://eln.excellgen.com/index.php?action=history&feed=atom&title=458-pLenti-CymR 458-pLenti-CymR - Revision history 2026-10-18T10:58:29Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=458-pLenti-CymR&diff=2891&oldid=prev Yingying.lu at 19:57, 29 December 2011 2011-12-29T19:57:58Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 458-pLenti-CymR&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Subloning of 458-pLenti-CymR&amp;nbsp;from 457 pET28b-CymR to 152&amp;nbsp;pLenti-Plus <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2011-12-16---12-23 <br /> <br /> &#039;&#039;&#039;Notebook:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:458&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Subcloning ===<br /> <br /> Date: 2011-12-22 <br /> <br /> [[Image:458+478+491 clone 12-16-2011.JPG]]&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; style=&quot;width: 355px; height: 96px;&quot;<br /> |-<br /> | band&lt;br&gt; <br /> | band 1&lt;br&gt; <br /> | band2&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | 457 pET28b-CymR&lt;br&gt; <br /> | 152 pLenti-Plus &lt;br&gt;<br /> |-<br /> | digest enzyme&lt;br&gt; <br /> | BamH1 + Xho1&lt;br&gt; <br /> | BamH1 + Xho1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> [[Image:458 insert-2 12-20-2011.JPG]]&lt;br&gt; <br /> <br /> [[Image:458 insert 12-20-2011.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> combine insert above together&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2011-12-23&lt;br&gt; <br /> <br /> Make miniprep and digest with BamH1 + Xho1&lt;br&gt; <br /> <br /> [[Image:458 digest detect 12-23-2011.JPG]] <br /> <br /> &amp;nbsp;8 clonies &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary ==<br /> <br /> &#039;&#039;&#039;sequence result: 414-6 right&#039;&#039;&#039;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> 414-6 right&lt;br&gt; &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div> Yingying.lu