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459-PCMVK-Gluc-PKA-Alpha - Revision history
2026-10-18T17:37:08Z
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Yingying.lu at 20:28, 3 January 2012
2012-01-03T20:28:48Z
<p></p>
<p><b>New page</b></p><div>Cloning of 459-pAAV-CMV-RFP <br />
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Purpose: to PCMVK-Gluc-PKA-Alpha by PCR<br> <br />
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Date: 2011-12-6---- <br />
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Notebook:Lu yingying <br />
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Page: <br />
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Clone ID:459 <br />
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Experimenters: lu yingying<br>Results<br> <br />
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[[Image:459 PCR 12-6-2011.JPG]]<br> <br />
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PCR using pLentiGP-Gluc-PKAalpha 300 as template and the following primers <br />
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Gluc-Xba1-F<br>atcga TCTAGA CCGGCCACCATGGGAGTCAAAG <br />
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PKA-6His-BamH1-R<br>(gcaaggagttttctgagttt CTC GAG CAT CAC CAT CAC CAT CAC TAA GGA TCC aaatag)<br>[[Image:459 clone 12-6-2011.JPG]]<br> <br />
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Cut PCR product with Xba1 + BamH1, clone into pCMVK-StrepII 193 (Xba1 + BamH1)<br> <br />
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[[Image:459 digest detect 12-12-2011.JPG]]<br> <br />
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digest detect: Xba1 + BamH1<br> --[[User:Yingying.lu|Yingying.lu]] 13:08, 12 December 2011 (EST) <br />
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<br><br />
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Date: 2011-12-20----<br />
<br />
Notebook:Lu yingying<br />
<br />
Page:<br />
<br />
Clone ID:459<br />
<br />
Experimenters: lu yingying<br>Results<br><br />
<br />
<br><br />
<br />
[[Image:459+493_PCR_12-19-2011.JPG]]<br><br />
<br />
PCR using pLentiGP-Gluc-PKAalpha 300 as template and the following primers<br />
<br />
Gluc-Xba1-F<br>atcga TCTAGA CCGGCCACCATGGGAGTCAAAG<br />
<br />
PKA-6His-BamH1-R<br>(gcaaggagttttctgagttt CTC GAG CAT CAC CAT CAC CAT CAC TAA GGA TCC aaatag)<br><br />
<br />
[[Image:169+323+459_clone_12-29-2011.JPG]]<br><br />
<br />
Cut PCR product with Xba1 + BamH1, clone into pCMVK-StrepII 193 (Xba1 + BamH1)<br><br />
<br />
[[Image:459_digest_detect_1-3-2012.JPG]]<br><br />
<br />
digest detect: Xba1 + BamH1<br><br />
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<br><br />
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[[Category:Cloning]]</div>
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