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461-pAAV-CMV-Cre-mKate2 - Revision history
2026-10-18T11:51:23Z
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Yingying.lu at 16:37, 1 December 2011
2011-12-01T16:37:10Z
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<p><b>New page</b></p><div>Cloning of pAAV-CMV-Cre-mKate2 <br />
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Purpose: to clone gene synthesis to 284 pAAV-CMV-mkate2 <br />
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Date: 2011-11-29 <br />
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Notebook:Lu yingying <br />
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Page: <br />
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Clone ID:461 <br />
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Experimenters: lu yingying<br>Results<br> <br />
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[[Image:454 PCR 11-18-2011.JPG]]<br> <br />
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PCR the same to 454 PCR <br> <br />
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Cre-EcoR1-F <br />
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aacta gaattc gccacc ATGGGCCATCACCATCACCATCACG <br />
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Cre-BglII-R<br>(CGCCTGCTGGAAGATGGCGAT ggA GAT CT atcag )<br>ctgatAGATCTccATCGCCATCTTCCAGCAGGCG <br />
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PCR using pTriEx-HTNC 298 as template for PCR,---&gt; 1158 bp<br> <br />
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[[Image:457+461 clone 11-29-2011.JPG]]<br> <br />
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digest result<br> <br />
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band 3 show PCR product digest with EcoR1 + BglII<br>band 4 show&nbsp; vector 284 pAAV-CMV-mkate2 with EcoR1 + BamH1<br />
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[[Image:461+493_digest_detect_12-1-2011.JPG]]<br />
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digest detect:EcoR1+Xho1<br><br> <br />
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[[Category:Cloning]]</div>
Yingying.lu