https://eln.excellgen.com/index.php?action=history&feed=atom&title=468-pAd5.CMV-Cre-EGFP 2026-10-18T19:24:23Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=468-pAd5.CMV-Cre-EGFP&diff=3134&oldid=prev 2012-01-23T20:14:26Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 468-pAd5.CMV-Cre-EGFP&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 468-pAd5.CMV-Cre-EGFP by PCR&lt;br&gt; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-1-18-- <br /> <br /> &#039;&#039;&#039;Notebook:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:468&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039;&lt;br&gt; ==<br /> <br /> [[Image:468 PCR 1-18-2012.JPG]]&lt;br&gt; <br /> <br /> AAV8.2 (not 2) - Cre-EGFP as template&lt;br&gt; <br /> <br /> Cre-EcoR1-F2+EGFP-Xho1-R&lt;br&gt; <br /> <br /> [[Image:468_clone_1-19-2012.JPG]]&lt;br&gt;<br /> <br /> band 1 insert&lt;br&gt;<br /> <br /> [[Image:468_vector_+257_clone_1-20-2012.JPG]]&lt;br&gt;<br /> <br /> band 1 vector: pAd5-CMV-MCS 280&lt;br&gt; <br /> <br /> EcoR1+Xho1 &lt;br&gt;<br /> <br /> === Subcloning ===<br /> <br /> Date: &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2012-1-23&lt;br&gt; <br /> <br /> Make miniprep and digest with&amp;nbsp; EcoR1+Xho1&lt;br&gt; <br /> <br /> [[Image:468 digest detect 1-23-2012.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> === ===<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div>

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