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470-pAAV-CMV-RFP - Revision history
2026-10-18T15:01:52Z
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Luyingying at 18:34, 27 October 2011
2011-10-27T18:34:59Z
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<p><b>New page</b></p><div>Cloning of 470-pAAV-CMV-RFP <br />
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Purpose: to subclone from pET28b-TagRFP 114 to pAAV.CMV-MCS 254<br> <br />
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Date: 2011-10-20---- <br />
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Notebook:Lu yingying <br />
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Page: <br />
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Clone ID:470 <br />
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Experimenters: lu yingying<br>Results<br> <br />
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[[Image:470-pAAV-CMV-RFP-10-20-2011.JPG]]<br> <br />
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band show digest result: BamH1 + Xho1 NEB enzyme for 2 hours<br> <br />
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band 1 show 254-pAAV.CMV-MCS&nbsp; vector<br> <br />
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band 2 show 114 pET28b-TagRFP insert<br> <br />
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ligation RT for 3 hours <br />
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Restart <br />
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Date: 2011-10-21---- <br />
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Notebook:Lu yingying <br />
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Page: <br />
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Clone ID:470 <br />
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Experimenters: lu yingying<br>Results<br> <br />
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[[Image:470-pAAV-CMV-RFP-10-21-2011.JPG]]<br> <br />
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band show digest result: BamH1 + Xho1 NEB enzyme for 2 hours <br />
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band 1,2 show 254-pAAV.CMV-MCS vector <br />
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band 3,4 show 114 pET28b-TagRFP insert<br> <br />
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Date: 2011-10-25----<br />
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Notebook:Lu yingying<br />
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Page:<br />
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Clone ID:470<br />
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Experimenters: lu yingying<br>Results<br><br />
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[[Image:470+359+237cloning_10-25-2011.JPG]]<br><br />
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band 1 shows 114 pET28b-TagRFP insert<br><br />
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[[Image:470-pAAV-CMV-RFP-10-21-2011.JPG]]<br />
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band 4 show 254-pAAV.CMV-MCS vector <br><br />
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ligation 2 hours RT<br><br />
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[[Image:414+470+375+359_digest_detect10-27-2011.JPG]]<br><br />
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470 1-4 bands show digest detect:BamH1 + Xho1<br><br />
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[[Category:Cloning]]</div>
Luyingying