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471-pAAV.CMV-FLPe - Revision history
2026-10-18T13:48:44Z
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Yingying.lu at 17:06, 9 December 2011
2011-12-09T17:06:27Z
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<p><b>New page</b></p><div>Cloning of pAAV.CMV-FLPe <br />
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Purpose: to clone pAAV.CMV-FLPe by PCR <br />
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Date: 2011-12-7---- <br />
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Notebook:Lu yingying <br />
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Page: <br />
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Clone ID:471 <br />
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Experimenters: lu yingying<br>Results<br> <br />
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[[Image:457+471+477+500 PCR 12-5-2011.JPG|520x215px|457+471+477+500 PCR 12-5-2011.JPG]]<br> <br />
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gene synthesis<br> PCR1, 10 cycles<br> 1 + 18<br> 19 + 38<br> <br> PCR2 mixture of PCR1<br> <br> 1 and 38 as primers, 25 cycles<br> <br />
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[[Image:471 clone 12-7-2011.JPG]]<br> <br />
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digest with EcoR1 + BamH1<br>clone into EcoR1 + BamH1 digested pAAV.CMV-MCS, 254<br>ligate and transform STBL3<br> <br />
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[[Image:471+477_digest_detect_12-9-2011.JPG]]<br><br />
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digest detect: EcoR1 + BamH1<br><br />
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[[Category:Cloning]]</div>
Yingying.lu