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472-pAd5.CMV-MCS2 - Revision history
2026-10-18T18:53:49Z
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Xiaojing.Wang at 17:41, 26 March 2012
2012-03-26T17:41:25Z
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<p><b>New page</b></p><div>Cloning of pAd5.CMV-MCS2 (472)<br>Purpose: to clone pAd5.CMV-MCS2&nbsp;<br>Date: 2011-12-20 to 2012-1-5<br>Notebook: Xiaojing Wang<br>Page: 004148---004148<br>Clone ID: 472<br>Experimenters: Xiaojing Wang<br>Results <br />
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1 annealed oligos: &nbsp; <br />
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pAD5-MCS-T4 E7 CGGATCCtctagaGAATTCACCGGTAAGCTTCTCGAGCCTAGGGC <br>pAD5-MCS-B4 E8 GGCCGCctcgagCACGTGAAGCTTtctagaggatccACCGGTgaattcGGTAC <br />
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2 digest pAd5-CMV-EGFP(304) with Kpn1 + Not1, cut out vector. Control is 304 plasmid. <br />
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[[Image:304 digest 12.20.2011' s.JPG]] <br />
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Ligation, Transformation <br />
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Select 10 clones to mini-prep<br>472 plasmids are digested by Nde1+BamH1<br />
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[[Image:472 digest 1.5.2012.JPG]] <br />
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[[Category:Cloning]]</div>
Xiaojing.Wang