https://eln.excellgen.com/index.php?action=history&feed=atom&title=475-PET28b-pfu 2026-10-18T16:58:06Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=475-PET28b-pfu&diff=2859&oldid=prev 2011-12-27T14:02:55Z

<p></p> <p><b>New page</b></p><div>cloning of 47-PET28b-pfu <br /> <br /> Purpose: to clone PET28b-pfu by gene synthesis to PET28b&lt;br&gt; <br /> <br /> Date: 2011-11-29---- <br /> <br /> Notebook:Lu yingying <br /> <br /> Page: <br /> <br /> Clone ID:475 <br /> <br /> Experimenters: lu yingying&lt;br&gt;Results&lt;br&gt; <br /> <br /> [[Image:475 PCR11-29-2011.JPG]]&lt;br&gt; <br /> <br /> PCR&lt;br&gt; <br /> <br /> template: 381pSpeedET-Pfu&lt;br&gt; <br /> <br /> primer: Pfu-F2 + Pfu-KpnI-R&lt;br&gt; <br /> <br /> [[Image:475 clone 11-29-2011.JPG]]&lt;br&gt; <br /> <br /> digest: Nde1+ Kpn1 &lt;br&gt; <br /> <br /> band 1 digested PCR insert&lt;br&gt; <br /> <br /> band 2 digested pET28b&lt;br&gt; <br /> <br /> [[Image:361+475+480 digest detect12-5-2011.JPG]]&lt;br&gt; <br /> <br /> digest detect: Nde1+ Kpn1 &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> Date: 2011-12-12 <br /> <br /> Notebook:Lu yingying <br /> <br /> Page: <br /> <br /> Clone ID:477 <br /> <br /> Experimenters: lu yingying&lt;br&gt;Results &lt;br&gt; <br /> <br /> [[Image:475+477 clone 12-12-2011.JPG]]&lt;br&gt; <br /> <br /> digest: Nde1+ Kpn1 <br /> <br /> band 1 digested PCR insert <br /> <br /> band 2 digested pET28b&lt;br&gt; <br /> <br /> [[Image:475+477 digest 12-14-2011.JPG]]&lt;br&gt; <br /> <br /> digest detect: Nde1+ Kpn1 &lt;br&gt;&lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Cloning of 475-PET28b-pfu&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 475-PET28b-pfu by PCR&lt;br&gt; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2011-12-20 <br /> <br /> &#039;&#039;&#039;Notebook: 004061--&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:475&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;[[Image:475+477+493 clone 12-21-2011.JPG]]&#039;&#039;&#039;&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; style=&quot;width: 278px; height: 96px;&quot;<br /> |-<br /> | &lt;br&gt; <br /> | band1&lt;br&gt; <br /> | band2&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | PCR product&lt;br&gt; <br /> | PET28b&lt;br&gt;<br /> |-<br /> | digest&lt;br&gt; <br /> | Nde1+ Kpn1&lt;br&gt; <br /> | Nde1+ Kpn1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> === Subcloning ===<br /> <br /> Date:&amp;nbsp; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date:2011-12-23&lt;br&gt; <br /> <br /> Make miniprep and digest with Nde1+ Kpn1&lt;br&gt; <br /> <br /> [[Image:475+477_digest_detect_12-23-2011.JPG]]<br /> <br /> &amp;nbsp;&lt;br&gt; &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; <br /> <br /> == ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> === ===<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div>

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