https://eln.excellgen.com/index.php?action=history&feed=atom&title=477-PET30b-MMLV-N 477-PET30b-MMLV-N - Revision history 2026-10-18T14:10:14Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=477-PET30b-MMLV-N&diff=2858&oldid=prev Ruili.su at 14:01, 27 December 2011 2011-12-27T14:01:23Z <p></p> <p><b>New page</b></p><div>Cloning of 477-PET30b-MMLV-N <br /> <br /> Purpose: to clone PET30b-MMLV-N by PCR &lt;br&gt; <br /> <br /> Date: 2011-12-5---- <br /> <br /> Notebook:Lu yingying <br /> <br /> Page: <br /> <br /> Clone ID:477 <br /> <br /> Experimenters: lu yingying&lt;br&gt;Results <br /> <br /> [[Image:457+471+477+500 PCR 12-5-2011.JPG|409x169px|457+471+477+500 PCR 12-5-2011.JPG]]&lt;br&gt; <br /> <br /> Gene synthesis of fragment 1&amp;nbsp;:MMLV-1 to MMLV-30&lt;br&gt; <br /> <br /> [[Image:477+491 clone 12-6-2011.JPG]]&lt;br&gt; <br /> <br /> digest with Nde1 + Nco1, clone into Nde1 + Nco1 digested pET30b <br /> <br /> [[Image:471+477 digest detect 12-9-2011.JPG]] <br /> <br /> &lt;br&gt;&lt;br&gt; --[[User:Yingying.lu|Yingying.lu]] 15:14, 9 December 2011 (EST) <br /> <br /> &lt;br&gt; <br /> <br /> Date: 2011-12-12&lt;br&gt; <br /> <br /> ----<br /> <br /> Notebook:Lu yingying <br /> <br /> Page: <br /> <br /> Clone ID:477 <br /> <br /> Experimenters: lu yingying&lt;br&gt;Results &lt;br&gt; <br /> <br /> [[Image:475+477 clone 12-12-2011.JPG]]&lt;br&gt; <br /> <br /> digest with Nde1 + Nco1, clone into Nde1 + Nco1 digested pET30b &lt;br&gt; <br /> <br /> [[Image:475+477 digest 12-14-2011.JPG]] <br /> <br /> [[Image:475+477 digest again12-15-2011.JPG]] <br /> <br /> == &#039;&#039;&#039;Cloning of 477-PET30b-MMLV-N&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 477-PET30b-MMLV-N <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2011-12-20--- <br /> <br /> &#039;&#039;&#039;Notebook:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID: 477&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;[[Image:475+477+493 clone 12-21-2011.JPG]]&#039;&#039;&#039;&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; style=&quot;width: 279px; height: 96px;&quot;<br /> |-<br /> | &lt;br&gt; <br /> | band 3&lt;br&gt; <br /> | band 4&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | PCR product&lt;br&gt; <br /> | PET30b&lt;br&gt;<br /> |-<br /> | digest &lt;br&gt; <br /> | Nde1 + Nco1&lt;br&gt; <br /> | Nde1 + Nco1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> === Subcloning ===<br /> <br /> Date:&amp;nbsp; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2011-12-23&lt;br&gt; <br /> <br /> Make miniprep and digest with Nde1 + Nco1&lt;br&gt; <br /> <br /> [[Image:475+477_digest_detect_12-23-2011.JPG]]<br /> <br /> clone 2,3,5,6,7 may right, need to sequence &lt;br&gt; &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div> Ruili.su