https://eln.excellgen.com/index.php?action=history&feed=atom&title=478-pAd5.CMV-CuO-MCS 478-pAd5.CMV-CuO-MCS - Revision history 2026-10-18T10:34:46Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=478-pAd5.CMV-CuO-MCS&diff=2902&oldid=prev Yingying.lu at 20:57, 29 December 2011 2011-12-29T20:57:34Z <p></p> <p><b>New page</b></p><div>Cloning of pAd5.CMV-CuO-MCS <br /> <br /> Purpose: to clone pAd5.CMV-CuO-MCS form&amp;nbsp; 448 pLex-CMV-CuO to pAd5-CMV-MCS 280&lt;br&gt; <br /> <br /> Date: 2011-12-13--- <br /> <br /> Notebook:Lu yingying <br /> <br /> Page: <br /> <br /> Clone ID:478 <br /> <br /> Experimenters: lu yingying&lt;br&gt;Results&lt;br&gt; <br /> <br /> [[Image:478 clone 12-13-2011.JPG]]&lt;br&gt; <br /> <br /> pAd5-CMV-MCS 280 with Nde1 + BamH1, Digest 448 pLex-CMV-CuO with Nde1 + BamH1&lt;br&gt; <br /> <br /> &lt;br&gt; --[[User:Yingying.lu|Yingying.lu]] 12:36, 13 December 2011 (EST) <br /> <br /> == &#039;&#039;&#039;Cloning of 478-pAd5.CMV-CuO-MCS&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 448 pLex-CMV-CuO to pAd5-CMV-MCS 280 <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2011-12-27 <br /> <br /> &#039;&#039;&#039;Notebook:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID: 478&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Subcloning ===<br /> <br /> Date: 2011-12-27 <br /> <br /> [[Image:478_clone_12-27-2011.JPG]]&lt;br&gt;<br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; style=&quot;width: 438px; height: 96px;&quot;<br /> |-<br /> | &lt;br&gt;<br /> | band 1&lt;br&gt;<br /> | band 2&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | 448 pLex-CMV-CuO&lt;br&gt;<br /> | pAd5-CMV-MCS 280&lt;br&gt;<br /> |-<br /> | digest enzyme&lt;br&gt;<br /> | colspan=&quot;2&quot; | &amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; Nde1 + BamH1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2011-12-29&lt;br&gt; <br /> <br /> Make miniprep and digest with Nde1 + BamH1&lt;br&gt; <br /> <br /> [[Image:478+493_digest_detect_12-29-2011.JPG]]&amp;nbsp;&lt;br&gt; <br /> <br /> clone 1,2,3,5,6,7,8 may be right<br /> &lt;br&gt;<br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *Sequencing Result:&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> <br /> &lt;br&gt;<br /> === ===<br /> <br /> &lt;br&gt; --[[User:Yingying.lu|Yingying.lu]] 15:57, 29 December 2011 (EST)<br /> <br /> [[Category:Cloning]]</div> Yingying.lu