https://eln.excellgen.com/index.php?action=history&feed=atom&title=483-pET28b-TthPol 483-pET28b-TthPol - Revision history 2026-10-18T19:24:31Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=483-pET28b-TthPol&diff=9877&oldid=prev Paul.xu at 17:45, 31 July 2013 2013-07-31T17:45:55Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 483-pET28b-TthPol&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 483-pET28b-TthPol <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-7-26---2013-7-31<br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:483&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:483PCR 7-26-2013.JPG]]&lt;br&gt; <br /> <br /> PCR from ATCC Genomic DNA from Thermus thermophilus Strain DSM 7039 [HB27]&lt;br&gt;ATCC BAA-163D-5 <br /> <br /> TthPol-Nde1-F&lt;br&gt;TthPol-EcoR1-R&lt;br&gt; <br /> <br /> [[Image:483+1799clone 7-29-2013.JPG]]&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; width=&quot;200&quot;<br /> |-<br /> | lane&lt;br&gt; <br /> | 1&lt;br&gt; <br /> | 2&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | PCR product&lt;br&gt; <br /> | 1029,pHisGST&lt;br&gt;<br /> |-<br /> | enzymes&lt;br&gt; <br /> | colspan=&quot;2&quot; | &amp;nbsp;&amp;nbsp; EcoR1 + Nde1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-7-31<br /> <br /> Make miniprep and digest with EcoR1 + Nde1&lt;br&gt; <br /> <br /> [[Image:483digest_7-31-2013.JPG]]<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Paul.xu