https://eln.excellgen.com/index.php?action=history&feed=atom&title=491-PET30-Cre 491-PET30-Cre - Revision history 2026-10-18T10:30:45Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=491-PET30-Cre&diff=2889&oldid=prev Yingying.lu at 19:55, 29 December 2011 2011-12-29T19:55:17Z <p></p> <p><b>New page</b></p><div>Cloning of 491-PET30-Cre <br /> <br /> Purpose: to subclone from 298-pTriEx-HTNC to PET30b&lt;br&gt; <br /> <br /> Date: 2011-11-29---- <br /> <br /> Notebook:Lu yingying <br /> <br /> Page: <br /> <br /> Clone ID:491 <br /> <br /> Experimenters: lu yingying&lt;br&gt;Results&lt;br&gt; <br /> <br /> [[Image:491 subclone 11-29-2011.JPG]]&lt;br&gt; <br /> <br /> digest 298 pTriEx-HTNC with Nco1 +Xho1 --&amp;gt; insert, digest pET30b with Nco1 + Xho1 --&amp;gt;vector,&lt;br&gt;ligate and transform Top10&lt;br&gt; <br /> <br /> [[Image:457+491 digest detect 12-8-2011.JPG]]&lt;br&gt; <br /> <br /> digest detect: Nco1 + Xho1&lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Cloning of 491-PET30-Cre &#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; subcloning of 298-pTriEx-HTNC to PET30b <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2011-12-16---2011-12-21 <br /> <br /> &#039;&#039;&#039;Notebook:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:491&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;[[Image:458+478+491 clone 12-16-2011.JPG]]&#039;&#039;&#039; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; style=&quot;width: 284px; height: 80px;&quot;<br /> |-<br /> | &lt;br&gt; <br /> | band 5&lt;br&gt; <br /> | band 6&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | 298-pTriEx-HTNC&lt;br&gt; <br /> | PET30b &lt;br&gt;<br /> |-<br /> | digest&lt;br&gt; <br /> | Nco1 + Xho1&lt;br&gt; <br /> | Nco1 + Xho1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> === Subcloning ===<br /> <br /> Date: 2011-12-16 <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2011-12-21&lt;br&gt; <br /> <br /> Make miniprep and digest with Nco1 + Xho1&lt;br&gt; <br /> <br /> [[Image:491 digest detect 12-21-2011.JPG]]&amp;nbsp;&lt;br&gt; &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *Sequencing Result:491-3 right&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> 491-3 right&lt;br&gt; &lt;br&gt; --[[User:Yingying.lu|Yingying.lu]] 14:59, 21 December 2011 (EST) <br /> <br /> [[Category:Cloning]]</div> Yingying.lu