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492-pLVB-V5-EGFP - Revision history
2026-10-18T03:17:24Z
Revision history for this page on the wiki
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Luyingying: moved 492 to 492-pLVB-V5-EGFP
2011-11-15T18:33:55Z
<p>moved <a href="/index.php?title=492" class="mw-redirect" title="492">492</a> to <a href="/index.php?title=492-pLVB-V5-EGFP" title="492-pLVB-V5-EGFP">492-pLVB-V5-EGFP</a></p>
<p><b>New page</b></p><div>Purpose: . <br />
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Date: 2011-10-6---10-18 <br />
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Notebook: Yingying Lu <br />
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Page: <br />
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Clone ID:392 <br />
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Experimenters: Yingying Lu<br>Results <br />
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[[Image:Gateway cloning 391,392+393-10-18-2011.JPG]] <br />
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digest detect: NdeI+NheI <br />
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[[Image:Gateway detect391,392+393-10-21-2011.JPG]] <br />
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digest detect with&nbsp;:EcoR1+Nhe1 <br />
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[[Image:Gateway_digest10-24-2011.JPG]]<br> <br />
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digest with BamH1 <br />
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1 and 2 should be the right clone (linearied by BamH1). <br />
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3, 4, 5: do not know why there are two bands? <br />
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6, pLVP-Dest should should two bands <br />
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7. pLVB-V5-Dest should have two bands (good) <br> <br />
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[[Category:Cloning]]</div>
Luyingying