https://eln.excellgen.com/index.php?action=history&feed=atom&title=495-pAd52-CMV5-CuO-MCS 495-pAd52-CMV5-CuO-MCS - Revision history 2026-10-18T05:34:58Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=495-pAd52-CMV5-CuO-MCS&diff=3949&oldid=prev Paul.xu at 17:31, 20 April 2012 2012-04-20T17:31:51Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 495-pAd52-CMV5-CuO-MCS&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 495-pAd52-CMV5-CuO-MCS <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-4-17---2012-4-20 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:495&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === subcloning ===<br /> <br /> [[Image:495 clone 4-17-2012.JPG]]&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; style=&quot;width: 436px; height: 115px;&quot;<br /> |-<br /> | &lt;br&gt; <br /> | band 1&lt;br&gt; <br /> | band 2&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | pLex-CMV-CuO 448&lt;br&gt; <br /> | vector pAd52-mCMV-MCS 494&lt;br&gt;<br /> |-<br /> | enzymes &lt;br&gt; <br /> | colspan=&quot;2&quot; | &amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; Xba1 + EcoR1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2012-4-19 <br /> <br /> Make miniprep and digest with Xba1 + EcoR1 <br /> <br /> [[Image:495 diges detect 4-19-2012.JPG]][[Image:495+1092 digest detect 4-19-2012.JPG]] <br /> <br /> Date: 2012-4-20<br /> <br /> digest with Xho1 + EcoR1&lt;br&gt;<br /> <br /> [[Image:495_digest_detect_4-20-2012.JPG]]&lt;br&gt;<br /> <br /> &lt;br&gt; &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&amp;amp;amp;lt;br&amp;amp;amp;gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> [[Category:Cloning]]</div> Paul.xu