https://eln.excellgen.com/index.php?action=history&feed=atom&title=496-pAd52-CMV5-Cuo-MCS3 496-pAd52-CMV5-Cuo-MCS3 - Revision history 2026-10-18T14:36:48Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=496-pAd52-CMV5-Cuo-MCS3&diff=3980&oldid=prev Paul.xu at 17:57, 25 April 2012 2012-04-25T17:57:33Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 496-pAd52-CMV5-Cuo-MCS3&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 496-pAd52-CMV5-Cuo-MCS3 <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-4-23---2012-4-25 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:496&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:496_clone_4-23-2012.JPG]]&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; style=&quot;width: 307px; height: 115px;&quot;<br /> |-<br /> | &lt;br&gt; <br /> | band 1&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | pAd52-CMV5-MCS 495 &lt;br&gt;<br /> |-<br /> | enzymes &lt;br&gt; <br /> | colspan=&quot;2&quot; | &amp;nbsp;EcoR11 + Sal1&lt;br&gt;<br /> |}<br /> <br /> digest pAd52-CMV5-MCS 495 with EcoR11 + Sal1, ligate to annealed pAd52-CMV5-MCS3-T + pAd52-CMV5-MCS3-B&lt;br&gt;&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2012-4-25 <br /> <br /> Make miniprep and digest with Xho1+ Kpn1&amp;nbsp; and&amp;nbsp;&amp;nbsp; HindIII + Pst1 <br /> <br /> [[Image:496_digest_detect_4-25-2012.JPG]]&lt;br&gt; <br /> <br /> clone 3 may be right<br /> &lt;br&gt;<br /> &lt;br&gt; <br /> <br /> &lt;br&gt; &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&amp;amp;amp;lt;br&amp;amp;amp;gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> [[Category:Cloning]]</div> Paul.xu