https://eln.excellgen.com/index.php?action=history&feed=atom&title=497-pAd52-CMV5-CuO-DEST-C6His 497-pAd52-CMV5-CuO-DEST-C6His - Revision history 2026-10-18T17:28:51Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=497-pAd52-CMV5-CuO-DEST-C6His&diff=4140&oldid=prev Paul.xu at 15:23, 7 May 2012 2012-05-07T15:23:15Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 497-pAd52-CMV5-CuO-DEST-C6His&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 497-pAd52-CMV5-CuO-DEST-C6His <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-4-26---2012-4-30 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:497&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === subcloning ===<br /> <br /> [[Image:497clone 2nd digest 4-26-2012.JPG]]&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; style=&quot;width: 391px; height: 98px;&quot;<br /> |-<br /> | &lt;br&gt; <br /> | band 1&lt;br&gt; <br /> | band 2&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | pAd5-CMV-Dest-His 374&lt;br&gt; <br /> | pAd52-CMV5-Cuo-MCS3, 496&lt;br&gt;<br /> |-<br /> | enzymes &lt;br&gt; <br /> | Kpn1 + Xho1&lt;br&gt; <br /> | Kpn1 + Sal1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === &#039;&#039;&#039;Colony Screening&#039;&#039;&#039; ===<br /> <br /> Date: 2012-4-30 <br /> <br /> Make miniprep and digest with EcoR1+Mlu1 <br /> <br /> [[Image:497+1068 digest detect 4-30-2012.JPG]] <br /> <br /> no insert <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; <br /> <br /> == ==<br /> <br /> *&amp;amp;amp;amp;lt;br&amp;amp;amp;amp;gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &amp;amp;lt;br&amp;amp;gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Cloning of 497-pAd52-CMV5-CuO-DEST-C6His&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 497-pAd52-CMV5-CuO-DEST-C6His <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-5-2---2012-5-4 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:497&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === subcloning ===<br /> <br /> [[Image:497]][[Image:497+1073+1092 clone 5-2-2012.JPG]]&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; style=&quot;width: 391px; height: 98px;&quot;<br /> |-<br /> | &lt;br&gt; <br /> | band 1&lt;br&gt; <br /> | band 2&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | pAd5-CMV-Dest-His 374&lt;br&gt; <br /> | pAd52-CMV5-Cuo-MCS3, 496&lt;br&gt;<br /> |-<br /> | enzymes &lt;br&gt; <br /> | Kpn1 + Xho1&lt;br&gt; <br /> | Kpn1 + Sal1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === &#039;&#039;&#039;Colony Screening&#039;&#039;&#039; ===<br /> <br /> Date: 2012-5-4 <br /> <br /> Make miniprep and digest with EcoR1+Mlu1 <br /> <br /> [[Image:497+1073 digest detect 5-4-2012.JPG|369x194px]][[Image:497_digest_detect_5-7-2012.JPG|350x195px]] <br /> <br /> clone 5,8 may be right&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; <br /> <br /> == ==<br /> <br /> *&amp;amp;amp;amp;lt;br&amp;amp;amp;amp;gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &amp;amp;lt;br&amp;amp;gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> [[Category:Cloning]]</div> Paul.xu