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500-PET30b-MMLV-C - Revision history
2026-10-18T17:21:41Z
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Yingying.lu at 21:08, 8 December 2011
2011-12-08T21:08:04Z
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<p><b>New page</b></p><div>Cloning of PET30b-MMLV-C <br />
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Purpose: to clone PET30b-MMLV-Cby PCR <br />
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Date: 2011-12-6 <br />
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Notebook:Lu yingying <br />
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Page: <br />
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Clone ID:500 <br />
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Experimenters: lu yingying<br>Results<br> <br />
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[[Image:457+471+477+500 PCR 12-5-2011.JPG|394x163px|457+471+477+500 PCR 12-5-2011.JPG]]<br> <br />
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Gene synthesis of fragment 1,MMLV-31 to MMLV-52<br> <br />
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[[Image:493+500 clone 12-6-2011.JPG]]<br> <br />
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digest with Nco1 + Xho1, clone into Nco1 + Xho1 digested pET3ob<br> <br />
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[[Image:500_digest_detect_12-8-2011.JPG]]<br><br />
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digest detect: Nco1 + Xho1<br><br />
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[[Category:Cloning]]</div>
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