https://eln.excellgen.com/index.php?action=history&feed=atom&title=501-pET30b-MMLV
501-pET30b-MMLV - Revision history
2026-10-18T15:41:55Z
Revision history for this page on the wiki
MediaWiki 1.44.0
https://eln.excellgen.com/index.php?title=501-pET30b-MMLV&diff=3069&oldid=prev
Ruili.su at 19:05, 12 January 2012
2012-01-12T19:05:55Z
<p></p>
<p><b>New page</b></p><div>== '''Cloning of ''''''501-pET30b-MMLV'''<br> <br />
<br />
'''Purpose:''' Subcloning of MMLV-C from pET30b-MMLV-C 500 pET30b-MMLV-N 477 <br />
<br />
'''Date: '''2012-1-9---- <br />
<br />
'''Notebook:''' <br />
<br />
'''Page:''' <br />
<br />
'''Clone ID:501''' <br />
<br />
'''Experimenters:''' yingying <br />
<br />
== '''Results''' ==<br />
<br />
=== Subcloning ===<br />
<br />
Date: 2012-1-9 <br />
<br />
[[Image:501 clone 1-9-2012.JPG]]<br> <br />
<br />
{| cellspacing="1" cellpadding="1" border="1" style="width: 329px; height: 112px;"<br />
|-<br />
| <br> <br />
| band 1<br> <br />
| band2<br><br />
|-<br />
| <br> <br />
| pET30b-MMLV-C 500<br> <br />
| pET30b-MMLV-N 477<br><br />
|-<br />
| digest enzyme<br> <br />
| Nco1 + Xho1<br> <br />
| Nco1 + Xho1<br><br />
|}<br />
<br />
<br> <br />
<br />
<br> <br />
<br />
{| cellspacing="1" cellpadding="1" border="1" style="width: 7px; height: 23px;"<br />
|-<br />
| <br><br />
|}<br />
<br />
<br> <br />
<br />
<br> <br />
<br />
<br> <br />
<br />
=== Colony Screening ===<br />
<br />
Date:2012-1-11<br />
<br />
Make miniprep and digest with Nco1 + Xho1<br> <br />
<br />
[[Image:501_digest_detect_1-11-2012.JPG]]<br />
<br />
<br> <br />
<br />
<br> <br />
<br />
<br> <br />
<br />
== '''Result intepretation/Summary''' ==<br />
<br />
== '''Conclusion''' ==<br />
<br />
<br> <br />
<br />
=== ===<br />
<br />
-[[User:Yingying.lu|Yingying.lu]] 09:23, 3 January 2012 (EST)<br> <br />
<br />
[[Category:Cloning]]</div>
Ruili.su