https://eln.excellgen.com/index.php?action=history&feed=atom&title=512-pET8XHis-Taq 512-pET8XHis-Taq - Revision history 2026-10-18T09:55:47Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=512-pET8XHis-Taq&diff=2914&oldid=prev Yingying.lu at 14:23, 3 January 2012 2012-01-03T14:23:03Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 512-pET8XHis-Taq&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Subcloning of 512-pET8XHis-Taq from pET28b-Taq 176 to pET8XHis 286&lt;br&gt; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2011-12-15---- <br /> <br /> &#039;&#039;&#039;Notebook:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:512&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Subcloning ===<br /> <br /> Date: 2011-12-15 <br /> <br /> [[Image:512 subclone 12-16-2011.JPG]]&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; style=&quot;width: 414px; height: 80px;&quot;<br /> |-<br /> | &lt;br&gt; <br /> | band 1&lt;br&gt; <br /> | band2&lt;br&gt; <br /> | band 3&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | pET28b-Taq 176&lt;br&gt; <br /> | pET28b-Taq 176&lt;br&gt; <br /> | pET8XHis 286&lt;br&gt;<br /> |-<br /> | digest&lt;br&gt; <br /> | colspan=&quot;3&quot; | <br /> &amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; Nco1 + Sal1&lt;br&gt; <br /> <br /> |}<br /> <br /> wild type&lt;br&gt; <br /> <br /> [[Image:462 digest 12-27-2011.JPG]]&lt;br&gt; <br /> <br /> wild type&amp;nbsp;&amp;nbsp;:Wt-Taq-XuYan, 462&lt;br&gt; <br /> <br /> Nco1 + Sal1&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date:12-30-2011&lt;br&gt; <br /> <br /> Make miniprep and digest with Nco1 + Sal1&lt;br&gt; <br /> <br /> [[Image:475+512_digest_detect_12-30-2011.JPG]]<br /> <br /> <br /> <br /> &amp;nbsp;&lt;br&gt; &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> === ===<br /> <br /> &lt;br&gt; <br /> --[[User:Yingying.lu|Yingying.lu]] 09:23, 3 January 2012 (EST)<br /> [[Category:Cloning]]</div> Yingying.lu