https://eln.excellgen.com/index.php?action=history&feed=atom&title=519-pAD5.CAG-mMKOS
519-pAD5.CAG-mMKOS - Revision history
2026-10-18T10:37:00Z
Revision history for this page on the wiki
MediaWiki 1.44.0
https://eln.excellgen.com/index.php?title=519-pAD5.CAG-mMKOS&diff=3029&oldid=prev
Yingying.lu at 18:06, 10 January 2012
2012-01-10T18:06:11Z
<p></p>
<p><b>New page</b></p><div>Cloning ofpAD5.CAG-mMKOS (519)<br>Purpose: to clone pAD5.CAG-mMKOS by subclone<br>Date: 2011-12-30 to 2012-1-5<br>Notebook: Xiaojing Wang<br>Page: 004147---004147<br>Clone ID: 519<br>Experimenters: Xiaojing Wang<br>Results&nbsp; <br />
<br />
Cut out KOSM from pLVX2MKOSiE 314 using Nde1 + BamH1,&nbsp;the insert is 6.5 kb. <br>Subclone into Nde1 + BamH1 digested pAd5-CMV-MCS 280. <br />
<br />
[[Image:314 280 digest 12.16.2011.JPG]] <br />
<br />
ligation, transformation <br />
<br />
select 5 clones to mini-prep <br />
<br />
519 plasmids are digested by Nde1+BamH1,519-1,-3,-4 plasmids may be correct.&nbsp; <br />
<br />
[[Image:519digest f 1.4.2012 s.JPG]] <br />
<br />
<br> <br />
<br />
519-1,-3,-4 plasmids are digested by xho1. <br><br />
<br />
[[Image:519digest xho1 1.5.2012 s.JPG]] <br />
<br />
[[Category:Cloning]]</div>
Yingying.lu