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521-pCDNA3.1-E1B-19K - Revision history
2026-10-18T08:49:50Z
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Yingying.lu at 15:32, 24 January 2012
2012-01-24T15:32:38Z
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<p><b>New page</b></p><div>Cloning of pCDNA3.1-E1B-19K (521) <br />
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Purpose: to clone pCDNA3.1-E1B-19K by Gene Synthesis <br />
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Date: 2012-1-18 to 2012-1-23 <br />
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Notebook: Xiaojing Wang <br />
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Page: 004163---004164 <br />
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Clone ID: 521 <br />
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Experimenters: Xiaojing Wang <br />
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Results <br> <br />
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[[Image:521 PCR 1.6.2012.JPG|109x317px|521 PCR 1.6.2012.JPG]]<br> <br />
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digest:<br> <br />
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[[Image:521 524 vector digest 1.18.2012s.JPG]]<br> <br />
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digest PCR product and vector with EcoR1 +Xho1,run gel <br />
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the left band shows 521 insert, the right picture shows 335 vector (pCDNA3.1, 335) <br />
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gel purify, ligation, transformation <br />
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Colony Screening <br> <br />
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[[Image:521 digest 1.23.2012.JPG]]<br> <br />
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Select 5 clones to mini-prep, digestd by EcoR1 and Xho1. 521-1,-3,-4,-5 may be correct. Control is 521-1.<br> <br />
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Select 521-1 to sequence.<br> <br />
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[[Category:Cloning]]</div>
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