https://eln.excellgen.com/index.php?action=history&feed=atom&title=522-pCDNA3.1-Bcl-X
522-pCDNA3.1-Bcl-X - Revision history
2026-10-18T08:47:38Z
Revision history for this page on the wiki
MediaWiki 1.44.0
https://eln.excellgen.com/index.php?title=522-pCDNA3.1-Bcl-X&diff=3150&oldid=prev
Yingying.lu at 15:37, 24 January 2012
2012-01-24T15:37:27Z
<p></p>
<p><b>New page</b></p><div>Cloning of pCDNA3.1-Bcl-X (522) <br />
<br />
Purpose: to clone pCDNA3.1-Bcl-X by Gene Synthesis <br />
<br />
Date: 2012-1-18 to 2012-1-23 <br />
<br />
Notebook: Xiaojing Wang <br />
<br />
Page: 004163---004164 <br />
<br />
Clone ID: 522 <br />
<br />
Experimenters: Xiaojing Wang <br />
<br />
Results <br> <br />
<br />
[[Image:522 PCR 1.18.2012.JPG|111x254px|522 PCR 1.18.2012.JPG]]<br> <br />
<br />
digest:<br> <br />
<br />
[[Image:522 and vector digest 1.19.2012.JPG]]<br> <br />
<br />
digest PCR product and vector with Nhe1 +Xho1,run gel <br />
<br />
the left band shows 522 insert, the right band shows 335 vector (pCDNA3.1, 335) <br />
<br />
gel purify, ligation, transformation <br />
<br />
<br>Colony Screening <br> <br />
<br />
[[Image:522 digest 1.23.2012 s.JPG]]<br> <br />
<br />
Select 5 clones to mini-prep, digestd by Nhe1 and Xho1. 522-2, -3, -5 may be correct. Control is 522-1. <br />
<br />
Select 522-3 to sequence.<br> <br />
<br />
<br> <br />
<br />
<br> <br />
<br />
[[Category:Cloning]]</div>
Yingying.lu