https://eln.excellgen.com/index.php?action=history&feed=atom&title=524-pCDNA3.1-E1A 2026-10-18T08:06:00Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=524-pCDNA3.1-E1A&diff=3158&oldid=prev 2012-01-24T15:55:50Z

<p>Created page with &quot;Cloning of pCDNA3.1-E1A (524) Purpose: to clone pCDNA3.1-E1A by Gene Synthesis Date: 2012-1-18 to 2012-1-23 Notebook: Xiaojing Wang Page: 004163---004164 Clone ID: 524 Expe...&quot;</p> <p><b>New page</b></p><div>Cloning of pCDNA3.1-E1A (524)<br /> <br /> Purpose: to clone pCDNA3.1-E1A by Gene Synthesis<br /> <br /> Date: 2012-1-18 to 2012-1-23<br /> <br /> Notebook: Xiaojing Wang<br /> <br /> Page: 004163---004164<br /> <br /> Clone ID: 524<br /> <br /> Experimenters: Xiaojing Wang<br /> <br /> Results &lt;br&gt;<br /> <br /> [[Image:524 PCR 1.6.2012S.JPG|114x296px]]&lt;br&gt;<br /> <br /> digest:&lt;br&gt;<br /> <br /> [[Image:521 524 vector digest 1.18.2012s.JPG]]&lt;br&gt;<br /> <br /> digest PCR product and vector with EcoR1 +Xho1,run gel<br /> <br /> the left band shows 524 insert, the right picture shows 335 vector (pCDNA3.1, 335)<br /> <br /> gel purify, ligation, transformation<br /> <br /> &lt;br&gt;Colony Screening &lt;br&gt;<br /> <br /> [[Image:524 digest 1.23.2012.JPG]]&lt;br&gt;<br /> <br /> Select 5 clones to mini-prep, digestd by EcoR1 and Xho1. 524-1,-3,-4,-5 may be correct. &lt;br&gt;<br /> <br /> Select 524-1 to sequence.&lt;br&gt;<br /> <br /> &lt;br&gt;<br /> <br /> &lt;br&gt;<br /> <br /> [[Category:Cloning]]</div>

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