Paul.xu at 19:34, 25 May 2012

2012-05-25T19:34:44Z

New page

== '''Cloning of 527-pLVX-EF1-Cre-IRES-EGFP''' ==

'''Purpose:''' Cloning of 527-pLVX-EF1-Cre-IRES-EGFP

'''Date: '''2012-5-23---2012-5-25

'''Notebook:0'''

'''Page:'''

'''Clone ID:527'''

'''Experimenters:''' yingying

== '''Results''' ==

=== subcloning ===

[[Image:527 clone 5-23-2012.JPG]] 

{| cellspacing="1" cellpadding="1" border="1" style="width: 392px; height: 80px;"
|-
| 
| band 1 
| band 2 
|-
| 
| 414 pAd5-CMV-Cre 
| 534 pLVX-EF1-IRES-EGFP 
|-
| enzymes 
| colspan="2" |            EcoR1 + Xho1 
|}

 

{| cellspacing="1" cellpadding="1" border="1"
|-
| 
|}

=== Colony Screening ===

Date: 2012-5-25

Make miniprep and digest with EcoR1 + Xho1 

[[Image:527_digest_detect_5-25-2012.JPG]] 

 

== '''Result intepretation/Summary''' ==

*&lt;br&gt;

== '''Conclusion''' ==

&lt;br&gt;

{| cellspacing="1" cellpadding="1"
|-
| 
|}

&lt;br&gt;

{| cellspacing="1"
|-
| 
|}

 

{| cellspacing="1"
|-
| 
|}

 

{|
|-
| 
|}

[[Category:Cloning]]

527-pLVX-EF1-Cre-IRES-EGFP - Revision history

Paul.xu at 19:34, 25 May 2012