https://eln.excellgen.com/index.php?action=history&feed=atom&title=528-pLVX-IRES-EGFP 528-pLVX-IRES-EGFP - Revision history 2026-10-18T12:09:19Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=528-pLVX-IRES-EGFP&diff=3004&oldid=prev Ruili.su at 17:00, 9 January 2012 2012-01-09T17:00:19Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 528-pLVX-IRES-EGFP&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of pLVX-IRES-EGFP by PCR <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2011-12-30--- <br /> <br /> &#039;&#039;&#039;Notebook:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:528&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> [[Image:528 PCR 12-30-2011.JPG]]&lt;br&gt; <br /> <br /> Primers&lt;br&gt;IRES-Xho1-F&lt;br&gt; Xho1&lt;br&gt;atcgca ctcgag TACGTAAATTCCGCCCCTCTC <br /> <br /> IRES-Mlu1-R&lt;br&gt;(CATGGACGAGCTGTACAAGTAA ACGCGT acgcatc)&lt;br&gt;gatgcgtACGCGTTTACTTGTACAGCTCGTCCATG <br /> <br /> PCR with IRES-Xho1-F + IRES-Mlu1-R using&amp;nbsp;pAd5-CMV-2-IRES-GFP 242 as template&lt;br&gt; <br /> <br /> [[Image:528 clone 1-4-2012.JPG]]&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; style=&quot;width: 381px; height: 112px;&quot;<br /> |-<br /> | &lt;br&gt; <br /> | band 1&lt;br&gt; <br /> | band 2&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | PCR product&lt;br&gt; <br /> | pLVXPlus2-IRES-mCherry, 174&lt;br&gt;<br /> |-<br /> | digest enzyme&lt;br&gt; <br /> | colspan=&quot;2&quot; | &amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; Xho1+Mlu1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> === Subcloning ===<br /> <br /> Date:&amp;nbsp; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2012-1-6&lt;br&gt; <br /> <br /> Make miniprep and digest with Xho1+Mlu1&lt;br&gt; <br /> <br /> [[Image:493+528 digest detect 1-6-2012.JPG]] <br /> <br /> transfection result: 528-1<br /> <br /> [[Image:2012 1 7 transfection reagents.jpg|474x351px]]<br /> <br /> &amp;nbsp;&lt;br&gt; &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *Sequencing Result:&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> Restriction digestion &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === ===<br /> <br /> &lt;br&gt; --[[User:Yingying.lu|Yingying.lu]] 09:21, 3 January 2012 (EST) <br /> <br /> [[Category:Cloning]]</div> Ruili.su