https://eln.excellgen.com/index.php?action=history&feed=atom&title=529-pLVX-mIL10-IRES-EGFP 529-pLVX-mIL10-IRES-EGFP - Revision history 2026-10-18T05:51:38Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=529-pLVX-mIL10-IRES-EGFP&diff=3194&oldid=prev Ruili.su: Created page with "'''Purpose:''' Subcloning of 529-pLVX-mIL10-IRES-EGFP by PCR<br> '''Date: '''2012-1-27---- '''Notebook:''' '''Page:''' '''Clone ID:529''' '''Experimenters:''' yingying ..." 2012-01-31T19:30:05Z <p>Created page with &quot;&#039;&#039;&#039;Purpose:&#039;&#039;&#039; Subcloning of 529-pLVX-mIL10-IRES-EGFP by PCR&lt;br&gt; &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-1-27---- &#039;&#039;&#039;Notebook:&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone ID:529&#039;&#039;&#039; &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying ...&quot;</p> <p><b>New page</b></p><div>&#039;&#039;&#039;Purpose:&#039;&#039;&#039; Subcloning of 529-pLVX-mIL10-IRES-EGFP by PCR&lt;br&gt; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-1-27---- <br /> <br /> &#039;&#039;&#039;Notebook:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:529&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Cloning ===<br /> <br /> Date: 2012-1-28 <br /> <br /> [[Image:529+532_PCR_1-27-2012.JPG]]&lt;br&gt;<br /> <br /> PCR using mIL10-BamH1-F + mIL10-Xho1-R, template is pBluescript SK-mIL10, 530&lt;br&gt;<br /> <br /> [[Image:529+532_clone_1-27-2012.JPG]]&lt;br&gt;<br /> <br /> &lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; style=&quot;width: 414px; height: 80px;&quot;<br /> |-<br /> | &lt;br&gt; <br /> | band 1&lt;br&gt; <br /> | band2&lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | PCR product&lt;br&gt; <br /> | pLVX-IRES-EGFP, 528&lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | digest&lt;br&gt; <br /> | colspan=&quot;3&quot; | <br /> &amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; BamH1 + Xho1&lt;br&gt; <br /> <br /> |}<br /> <br /> &lt;br&gt;<br /> <br /> &lt;br&gt;<br /> <br /> === Colony Screening ===<br /> <br /> Date:2012-1-31&lt;br&gt; <br /> <br /> Make miniprep and digest with BamH1 + Xho1&lt;br&gt; <br /> <br /> [[Image:529_digest_detect_1-31-2012.JPG]]&lt;br&gt; <br /> <br /> clone 3 may be right, need to sequencing<br /> <br /> &lt;br&gt; <br /> <br /> &amp;nbsp;&lt;br&gt; &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> === ===<br /> <br /> &lt;br&gt; --[[User:Yingying.lu|Yingying.lu]] &lt;br&gt;<br /> <br /> [[Category:Cloning]]</div> Ruili.su