https://eln.excellgen.com/index.php?action=history&feed=atom&title=531-pLVXPlus3-IRES-mCherry 2026-10-18T19:24:50Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=531-pLVXPlus3-IRES-mCherry&diff=3722&oldid=prev 2012-04-02T17:35:52Z

<p></p> <p><b>New page</b></p><div>Cloning of pLVXPlus3-IRES-mCherry (531)&lt;br&gt;Purpose: to clone pLVXPlus3-IRES-mCherry&lt;br&gt;Date: 2012-3-27 to 2012-3-30&lt;br&gt;Notebook: Xiaojing Wang&lt;br&gt;Page: 004170,004210&lt;br&gt;Clone ID: 531&lt;br&gt;Experimenters: Xiaojing Wang&lt;br&gt;Results <br /> <br /> 1 Annealed oligos:&lt;br&gt; <br /> <br /> pLVXPlus3-F&lt;br&gt; <br /> <br /> pLVXPlus3-R&lt;br&gt; <br /> <br /> 2 Digest pLVXPlus2-IRES-mCherry (174) with EcoR1 + Xho1, cut out vector. &lt;br&gt; <br /> <br /> [[Image:174 digest 3.27.2012.JPG]]&lt;br&gt; <br /> <br /> Ligation, Transformation <br /> <br /> Select 5 clones to mini-prep&lt;br&gt;531 plasmids are digested by BamH1 + Mlu1 or Xba1 + Mlu1&lt;br&gt; <br /> <br /> [[Image:531 2digest 3.30.2012.JPG]]&lt;br&gt; <br /> <br /> 531-1,-5 may be correct.<br /> <br /> Select 531-1 to sequence.&lt;br&gt; <br /> <br /> [[Category:Cloning]]</div>

Xiaojing.Wang