https://eln.excellgen.com/index.php?action=history&feed=atom&title=533-pLVX-Cre-IRES-EGFP 533-pLVX-Cre-IRES-EGFP - Revision history 2026-10-18T07:10:01Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=533-pLVX-Cre-IRES-EGFP&diff=3126&oldid=prev Ruili.su at 19:01, 20 January 2012 2012-01-20T19:01:47Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 533-pLVX-Cre-IRES-EGFP&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 533-pLVX-Cre-IRES-EGFP by subclone from 414 pAd5-CMV-Cre to 528 pLVX-IRES-EGFP&lt;br&gt; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-1-13-- <br /> <br /> &#039;&#039;&#039;Notebook:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:533&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039;&lt;br&gt; ==<br /> <br /> &lt;br&gt; <br /> <br /> === Subcloning ===<br /> <br /> Date:&amp;nbsp;2012-1-13 <br /> <br /> [[Image:533+534 clone 1-13-2012.JPG]]&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; style=&quot;width: 426px; height: 80px;&quot;<br /> |-<br /> | &lt;br&gt; <br /> | band 1&lt;br&gt; <br /> | band 2&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | 414 pAd5-CMV-Cre&lt;br&gt; <br /> | 528 pLVX-IRES-EGFP&lt;br&gt;<br /> |-<br /> | digestion enzymes&lt;br&gt; <br /> | colspan=&quot;2&quot; | &amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; EcoR1+Xho1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2012-1-17&lt;br&gt; <br /> <br /> Make miniprep and digest with&amp;nbsp; EcoR1+Xho1&lt;br&gt; <br /> <br /> [[Image:533+534 digest detect 1-17-2012.JPG]] <br /> <br /> clone 1,2,3,4 may right <br /> <br /> [[Image:533-1.JPG|157x160px]][[Image:533-2.JPG|169x159px]][[Image:533-3.JPG|164x159px]][[Image:533-4.JPG|172x159px]][[Image:533-5.JPG|157x157px]]<br /> <br /> transfection result: green fluorescense positive<br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> === ===<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div> Ruili.su