https://eln.excellgen.com/index.php?action=history&feed=atom&title=535-pAAV-CMV-DIO-sfGFP 2026-10-18T06:29:48Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=535-pAAV-CMV-DIO-sfGFP&diff=3176&oldid=prev 2012-01-30T19:02:40Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 535-pAAV-CMV-DIO-sfGFP&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 535-pAAV-CMV-DIO-sfGFP by genesythesis&lt;br&gt; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-1-24-- <br /> <br /> &#039;&#039;&#039;Notebook:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:535&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039;&lt;br&gt; ==<br /> <br /> &lt;br&gt; <br /> <br /> === Cloning ===<br /> <br /> Date:&amp;nbsp;2012-1-24 <br /> <br /> [[Image:535_PCR_1-24-2012.JPG]]&lt;br&gt;<br /> <br /> gene synthesis,&lt;br&gt;<br /> <br /> PCR1:DIO-sfGFP1 to DIO-sfGFP12&lt;br&gt;<br /> <br /> PCR2:DIO-sfGFP13 to DIO-sfGFP26&lt;br&gt;<br /> <br /> PCR: PCR1+PCR2 as template,&lt;br&gt;<br /> <br /> primer:sfGFP1+DIO-sfGFP26&lt;br&gt;<br /> <br /> [[Image:535_clone_1-25-2012.JPG]]&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; style=&quot;width: 422px; height: 83px;&quot;<br /> |-<br /> | &lt;br&gt; <br /> | band 1&lt;br&gt; <br /> | band 2&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | PCR product&lt;br&gt; <br /> | 254 pAAV.CMV-MCS&lt;br&gt;<br /> |-<br /> | digestion enzymes&lt;br&gt; <br /> | colspan=&quot;2&quot; | &amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; EcoR1 + BamH1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt;<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2012-1-30&lt;br&gt; <br /> <br /> Make miniprep and digest with&amp;nbsp; EcoR1 + BamH1&lt;br&gt; <br /> <br /> [[Image:323+534+535_digest_detect_1-30-2012.JPG]]&lt;br&gt; <br /> <br /> 535-1,2,3,4,5,6 may right ,need to sequence<br /> <br /> &lt;br&gt;<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> === ===<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div>

Ruili.su