https://eln.excellgen.com/index.php?action=history&feed=atom&title=542-pTracer-_BcCD 542-pTracer- BcCD - Revision history 2026-10-18T05:43:24Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=542-pTracer-_BcCD&diff=3187&oldid=prev Ruili.su at 19:21, 30 January 2012 2012-01-30T19:21:22Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 542-pTracer- BcCD&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 542-pTracer- BcCD by PCR&lt;br&gt; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-1-26-- <br /> <br /> &#039;&#039;&#039;Notebook:003719&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:542&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> Date:2012-1-26 <br /> <br /> [[Image:542,543,544_PCR_1-26-2012.JPG]]&lt;br&gt;<br /> <br /> PCR using BcCD 539 as template&lt;br&gt;<br /> <br /> Primer-F pBcCD-F, AGGGAGACCCAAGCTGGCTAGC gcc acc ATG TCT AAT AAT GCA CTC C&lt;br&gt;Primer-R pBcCD-R, ACGGGCCCTCTAGACTCGAGCGGCCGCTCACCTTTTATAATCAATTGC&lt;br&gt;<br /> <br /> [[Image:542,543,544_insert_1-27-2012.JPG]]&lt;br&gt;<br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; style=&quot;width: 311px; height: 80px;&quot;<br /> |-<br /> | &lt;br&gt;<br /> | band 1&lt;br&gt;<br /> | &lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | PCR insert&lt;br&gt;<br /> | &lt;br&gt;<br /> |-<br /> | digest enzymes&lt;br&gt;<br /> | Nhe1 + Not1&lt;br&gt;<br /> | &lt;br&gt;<br /> |}<br /> <br /> [[Image:542,543,544_vector_1-27-2012.JPG]]&lt;br&gt;<br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; style=&quot;width: 290px; height: 96px;&quot;<br /> |-<br /> | &lt;br&gt;<br /> | band &lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | pTracer.CMV/Bsd 538&lt;br&gt;<br /> |-<br /> | digest enzymes&lt;br&gt;<br /> | Nhe1 + Not1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt;<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2012-1-30 <br /> <br /> Make miniprep and digest with Nhe1 + Not1&lt;br&gt;<br /> <br /> [[Image:542+543+544_digest_detect_1-30-2012.JPG]]<br /> <br /> clone 1,2,3,4,5 may be right, need to sequence<br /> <br /> <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&amp;lt;br&amp;gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> === ===<br /> <br /> &amp;lt;br&amp;gt;&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> [[Category:Cloning]]</div> Ruili.su