https://eln.excellgen.com/index.php?action=history&feed=atom&title=544-pTracer-SPbacCD 544-pTracer-SPbacCD - Revision history 2026-10-18T05:43:07Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=544-pTracer-SPbacCD&diff=3189&oldid=prev Ruili.su: Created page with "== '''Cloning of 544-pTracer-SPbacCD''' == '''Purpose:''' Cloning of 544-pTracer-SPbacCD by PCR '''Date: '''2012-1-26-- '''Notebook:003719''' '''Page:''' '''Clone ID:54..." 2012-01-30T19:31:34Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 544-pTracer-SPbacCD&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 544-pTracer-SPbacCD by PCR &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-1-26-- &#039;&#039;&#039;Notebook:003719&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone ID:54...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 544-pTracer-SPbacCD&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 544-pTracer-SPbacCD by PCR <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-1-26-- <br /> <br /> &#039;&#039;&#039;Notebook:003719&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:544&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> Date:2012-1-26 <br /> <br /> [[Image:542,543,544 PCR 1-26-2012.JPG]] <br /> <br /> PCR template is SPbacCD 541<br /> <br /> Primer-F SPbacCD-F AGGGAGACCCAAGCTGGCTAGC gcc acc ATG GAC GCC ATG CTG CGA GG&lt;br&gt;Primer-R SPbacCD-R (GCTATTGACTACAAAAGGTGA GCGGCCGC TCGAGTCTAGAGGGCCCGT) ACGGGCCCTCTAGACTCGAGCGGCCGCTCACCTTTTGTAGTCAATAGC<br /> <br /> [[Image:542,543,544 insert 1-27-2012.JPG]]<br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; style=&quot;width: 288px; height: 80px;&quot;<br /> |-<br /> | &lt;br&gt;<br /> | band 3&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | PCR product&lt;br&gt;<br /> |-<br /> | digest enzymes&lt;br&gt;<br /> | Nhe1 + Not1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt;<br /> <br /> [[Image:542,543,544 vector 1-27-2012.JPG]]<br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; style=&quot;width: 281px; height: 96px;&quot;<br /> |-<br /> | &lt;br&gt;<br /> | band &lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | pTracer.CMV/Bsd 538&lt;br&gt;<br /> |-<br /> | digest enzymes&lt;br&gt;<br /> | Nhe1 + Not1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt;<br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |}<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2012-1-30 <br /> <br /> Make miniprep and digest with Nhe1 + Not1<br /> <br /> [[Image:542+543+544 digest detect 1-30-2012.JPG]] <br /> <br /> clone 1,2,3,4,5 may be right, need to sequence <br /> <br /> &lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> &lt;br&gt;<br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |}<br /> <br /> <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> [[Category:Cloning]]</div> Ruili.su