Paul.xu at 20:20, 20 February 2013

2013-02-20T20:20:52Z

New page

== '''Cloning of 553-567''' ==

'''Purpose:''' Cloning of 553-567

'''Date: '''2012-9-14---2012-9-20 

'''Notebook: '''

'''Page:'''

'''Clone ID: '''553---567

'''Experimenters: '''yingying lu

== '''Results''' ==

=== Cloning ===

Date: 2012-9-14

[[Image:553+560+561+563+564+566 PCR 9-14-2012.JPG]]

[[Image:554+557+558+559+562+565+567 PCR1+2 9-14-2012.JPG]]

[[Image:554+557+558+559+562+565+567 PCR 9-17-2012.JPG]]

 

2012-9-18 

clone 553(lane1)-567(lane13):Nde1+Xho1; plasmid 1254 as vector 

lane1 and 10 smear, so did not do the clone 553 and 564

[[Image:554-567 clone 9-19-2012.JPG]][[Image:554-567 vectror 9-19-2012.JPG]]

 

 

 

=== Colony Screening ===

2012-9-20

mini prep and digest with :Nde1+Xho1 

no insert

 

 

 

== '''Result intepretation/Summary''' ==

 

== '''Conclusion''' ==

 

== '''Cloning of 559-pET30b.Amp-Not1,567-pET30b.Amp-Not1''' ==

'''Purpose:''' Cloning of 559-pET30b.Amp-Not1,567-pET30b.Amp-Not1

'''Date: '''2012-10-9---2012-10-11

'''Notebook:0'''

'''Page:'''

'''Clone ID:559,567'''

'''Experimenters:''' yingying

== '''Results''' ==

=== cloning ===

[[Image:559+567+1097+1411 clone 10-9-2012.JPG]] 

 

{| cellspacing="1" cellpadding="1" border="1" style="width: 281px; height: 66px;"
|-
| 
| band 1,3 
| band 2,4 
|-
| 
| PCR product 
| pET30b.Amp 
|-
| enzymes 
| colspan="2" |             Nde1 + Xho1. 
|}

 

{| cellspacing="1" cellpadding="1" border="1"
|-
| 
|}

=== Colony Screening ===

Date: 2012-10-11

Make miniprep and digest with Nde1 + Xho1.

[[Image:559+567 digest detect 10-11-2012.JPG]] 

no insert ,only the vector self ligate

c 

== '''Result intepretation/Summary''' ==

*&lt;br&gt;

== '''Conclusion''' ==

&lt;br&gt;

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&lt;br&gt;

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== '''Cloning of 559-pET30b.Amp-Not1,567-pET30b.Amp-Not1''' ==

'''Purpose:''' Cloning of 559-pET30b.Amp-Not1,567-pET30b.Amp-Not1

'''Date: '''2012-10-15---2012-10-18

'''Notebook:0'''

'''Page:'''

'''Clone ID:559,567'''

'''Experimenters:''' yingying

== '''Results''' ==

=== cloning ===

[[Image:240+559+567+1248 clone 10-16-2012.JPG]] 

{| width="356" cellspacing="1" cellpadding="1" border="1"
|-
| 
| band 3,5 
| band 4,6 
|-
| 
| PCR product 
| 1026,pET28b-LambdaExo 
|-
| enzymes 
| colspan="2" |             Nde1 + Xho1. 
|}

 

{| cellspacing="1" cellpadding="1" border="1"
|-
| 
|}

=== Colony Screening ===

Date: 2012-10-18

Make miniprep and digest with Nde1 + Xho1.

[[Image:559+567+1248 digest detect 10-18-2012.JPG]] 

559clone 5,6,7,8 may be right ,

567 clone 4 may be right, 

c :1026pET28b-LambdaExo digested with Nde1 + Xho1 

== '''Result intepretation/Summary''' ==

*&lt;br&gt;

== '''Conclusion''' ==

&lt;br&gt;

{| cellspacing="1" cellpadding="1"
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&lt;br&gt;

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== '''Cloning of 554-pET28b-Nde1,560-pET28b-Kpn1''' ==

'''Purpose:''' Cloning of pET28b-Nde1,560-pET28b-Kpn1

'''Date: '''2013-2-13---2013-2-19

'''Notebook:0'''

'''Page:'''

'''Clone ID:554,560'''

'''Experimenters:''' yingying

== '''Results''' ==

=== cloning ===

[[Image:554+560+1540 clone 2-14-2013.JPG]] 

{| width="356" cellspacing="1" cellpadding="1" border="1"
|-
| 
| band 1,3 
| band 2,4 
|-
| 
| PCR product 
| ,pET28b 
|-
| enzymes 
| colspan="2" |             Nde1 + Xho1. 
|}

 

{| cellspacing="1" cellpadding="1" border="1"
|-
| 
|}

=== Colony Screening ===

Date: 2013-2-19

Make miniprep and digest with Nde1 + Xho1.

[[Image:554+560_digest_detect_2-19-2013.JPG]][[Image:554+1540_digest_detect_2-20-2013.JPG]] 

 

== '''Result intepretation/Summary''' ==

*&lt;br&gt;

== '''Conclusion''' ==

&lt;br&gt;

{| cellspacing="1" cellpadding="1"
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&lt;br&gt;

{| cellspacing="1"
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[[Category:Cloning]]

553-567 - Revision history

Paul.xu at 20:20, 20 February 2013