https://eln.excellgen.com/index.php?action=history&feed=atom&title=553-pET28b-Xho1 553-pET28b-Xho1 - Revision history 2026-10-19T11:05:25Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=553-pET28b-Xho1&diff=9172&oldid=prev Paul.xu at 14:21, 20 May 2013 2013-05-20T14:21:53Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 553-pET28b-Xho1&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 553-pET28b-Xho1 <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-5-6--2013-5-10 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:553&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:553 pcr1+pcr2 5-7-2013.JPG]] <br /> <br /> [[Image:553PCR 5-7-2013.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-5-10 <br /> <br /> Make miniprep and digest with Nde1 + Xho1 <br /> <br /> [[Image:553 digest 5-10-2013.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> {| cellspacing=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;fix of Cloning of 553-pET28b-Xho1&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 553-pET28b-Xho1 <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-5-14--2013-5-17 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:553&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:553pcr1+pcr2 5-14-2013.JPG]]&lt;br&gt; <br /> <br /> [[Image:553PCR 5-14-2013.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-5-17 <br /> <br /> Make miniprep and digest with Nde1 + Xho1 <br /> <br /> [[Image:553_digest_5-17-2013.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> {| cellspacing=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> [[Category:Cloning]]</div> Paul.xu