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555-pET28b-Hind111 - Revision history
2026-10-18T17:23:01Z
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Paul.xu at 14:11, 26 June 2013
2013-06-26T14:11:52Z
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<p><b>New page</b></p><div>== '''Cloning of 555-pET28b-Hind111''' ==<br />
<br />
'''Purpose:''' Cloning of 555-pET28b-Hind111 <br />
<br />
'''Date: '''2013-6-3--2013-6-7 <br />
<br />
'''Notebook:0''' <br />
<br />
'''Page:''' <br />
<br />
'''Clone ID:555''' <br />
<br />
'''Experimenters:''' yingying <br />
<br />
== '''Results''' ==<br />
<br />
=== cloning ===<br />
<br />
[[Image:555+556pcr1+pcr2 6-4-2013.JPG]] <br />
<br />
pcr1: 1-1 to 1-14<br />
<br />
pcr2:2-1 to 2-12<br />
<br />
[[Image:555+556PCR 6-5-2013.JPG]] <br />
<br />
pcr:1-1 to 2-12<br />
<br />
clone into pET28b Nde1+Xho1<br />
<br />
<br> <br />
<br />
<br> <br />
<br />
=== Colony Screening ===<br />
<br />
Date: 2013-6-7 <br />
<br />
Make miniprep and digest with: nde1+xho1 <br />
<br />
[[Image:555 digest 6-7-2013.JPG]]<br> <br />
<br />
== '''Result intepretation/Summary''' ==<br />
<br />
*&amp;amp;amp;amp;lt;br&amp;amp;amp;amp;gt;<br />
<br />
== '''Conclusion''' ==<br />
<br />
== '''fix of Cloning of 555-pET28b-Hind111''' ==<br />
<br />
'''Purpose:''' fix of Cloning of 555-pET28b-Hind111 <br />
<br />
'''Date: '''2013-6-20---2013-6-24 <br />
<br />
'''Notebook:0''' <br />
<br />
'''Page:''' <br />
<br />
'''Clone ID:555''' <br />
<br />
'''Experimenters:''' yingying <br />
<br />
== '''Results''' ==<br />
<br />
=== cloning ===<br />
<br />
[[Image:555+556+631pcr12 6-20-2013.JPG]]<br> <br />
<br />
555-4 above20120607 as template <br />
<br />
pcr1--primer:1-1 to 2-8 <br />
<br />
pcr2--primer:2-9 to 2-12 <br />
<br />
[[Image:555+556+631PCR 6-20-2013.JPG]] <br />
<br />
&nbsp;template:pcr1+pcr2 <br />
<br />
primer&nbsp;:1-1 and 2-12 <br />
<br />
[[Image:555+556 clone 6-21-2013.JPG]]<br> <br />
<br />
lane1: clone into vector 1225pET28b-T7-Endo1<br> <br />
<br />
nde1+xho1 <br />
<br />
<br> <br />
<br />
<br> <br />
<br />
=== Colony Screening ===<br />
<br />
Date: 2013-6-24 <br />
<br />
Make miniprep and digest with: nde1+xho1 <br />
<br />
[[Image:555+556+1619 digest 6-24-2013.JPG]]<br> <br />
<br />
== '''Result intepretation/Summary''' ==<br />
<br />
*&amp;amp;amp;amp;lt;br&amp;amp;amp;amp;gt;<br />
<br />
== '''Conclusion''' ==<br />
<br />
[[Category:Cloning]]</div>
Paul.xu