https://eln.excellgen.com/index.php?action=history&feed=atom&title=556-pET28b-Sma1 556-pET28b-Sma1 - Revision history 2026-10-18T08:12:44Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=556-pET28b-Sma1&diff=9542&oldid=prev Paul.xu at 18:37, 25 June 2013 2013-06-25T18:37:12Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 556-pET28b-Sma1&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 556-pET28b-Sma1 <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-6-3---2013-6-7 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:556&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:555+556pcr1+pcr2 6-4-2013.JPG]]&lt;br&gt; <br /> <br /> [[Image:555+556PCR 6-5-2013.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-6-7 <br /> <br /> Make miniprep and digest with:nde+xho1 <br /> <br /> [[Image:556+1704 digest 6-7-2013.JPG]] <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;fix of Cloning of 556-pET28b-Sma1&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; fix of Cloning of 556-pET28b-Sma1 <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-6-20---2013-6-24 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:556&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:555+556+631pcr12_6-20-2013.JPG]]&lt;br&gt;<br /> <br /> 556-4 above as template&lt;br&gt;<br /> <br /> 1-1 to 1-17&lt;br&gt;<br /> <br /> 1-16 to 1-20&lt;br&gt;<br /> <br /> [[Image:555+556+631PCR_6-20-2013.JPG]]&lt;br&gt;<br /> <br /> template:pcr1+pcr2&lt;br&gt;<br /> <br /> primer :1-1 and&amp;nbsp; 1-20&lt;br&gt;<br /> <br /> [[Image:555+556_clone_6-21-2013.JPG]]&lt;br&gt;<br /> <br /> lane 2: vector 1225pET28b-T7-Endo1&lt;br&gt;<br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-6-24 <br /> <br /> Make miniprep and digest with:nde+xho1 <br /> <br /> [[Image:555+556+1619_digest_6-24-2013.JPG]]<br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div> Paul.xu